PMID- 23402541
OWN - NLM
STAT- MEDLINE
DCOM- 20130628
LR  - 20211021
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 9
DP  - 2013 Feb 12
TI  - Effects of the breed, sex and age on cellular content and growth factor release 
      from equine pure-platelet rich plasma and pure-platelet rich gel.
PG  - 29
LID - 10.1186/1746-6148-9-29 [doi]
AB  - BACKGROUND: There is no information on the effects of the breed, gender and age 
      on the cellular content and growth factor (GF) release from equine pure-platelet 
      rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this 
      study were: 1) to compare the cellular composition of P-PRP with whole blood and 
      platelet poor plasma (PPP); 2) to compare the concentration of transforming GF 
      beta 1 (TGF-beta1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP 
      treated with non-ionic detergent (P-PRP+NID), P-PRG (activated with calcium 
      gluconate -CG-), PPP+NID, PPP gel (PPG), and plasma and; 3) to evaluate and to 
      correlate the effect of the breed, gender and age on the cellular and GF 
      concentration for each blood component. Forty adult horses, 20 Argentinean Creole 
      Horses (ACH) and, 20 Colombian Creole Horses (CCH) were included. Data were 
      analyzed by parametric (i.e.: t-test, one way ANOVA) and non parametric 
      (Kruskal-Wallis test, Wilcoxon test) tests. Correlation analysis was also 
      performed by using the Spearman and Pearson tests. A p </= 0.05 was set as 
      significant for all tests. All the blood components were compared for platelet 
      (PLT), leukocyte (WBC), TGF-beta1 and PDGF-BB concentrations. The effect of the 
      breed, gender and age on these variables was analyzed. A P </= 0.05 was accepted as 
      significant for all the tests. RESULTS: PLT counts were 1.8 and 0.6 times higher 
      in P-PRP than in whole blood and PPP, respectively; WBC counts were 0.5 and 0.1 
      times lower in P-PRP, in comparison with whole blood and PPP, respectively. 
      TGF-beta1 and PDGF-BB concentrations were 2.3 and 262 times higher, respectively, in 
      P-PRG than in plasma, and 0.59 and 0.48 times higher, respectively, in P-PRG than 
      in PPG. P-PRG derived from CCH females or young horses presented significantly (P 
      < 0.001) higher PDGF-BB concentrations than P-PRG derived from ACH males or older 
      horses. CONCLUSIONS: Our results indicated that P-PRP obtained by a manual method 
      was affected by intrinsic factors such as the breed, gender and age. Equine 
      practitioners should be aware that cellular and GF release from P-PRP/P-PRG could 
      change according with the intrinsic variables associated with a patient in 
      particular.
FAU - Giraldo, Carlos E
AU  - Giraldo CE
AD  - Grupo de Investigacion Terapia Regenerativa, Departamento de Salud Animal, 
      Universidad de Caldas, Manizales, Colombia.
FAU - Lopez, Catalina
AU  - Lopez C
FAU - Alvarez, Maria E
AU  - Alvarez ME
FAU - Samudio, Ismael J
AU  - Samudio IJ
FAU - Prades, Marta
AU  - Prades M
FAU - Carmona, Jorge U
AU  - Carmona JU
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130212
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Protein Isoforms)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Blood Platelets/*metabolism/physiology
MH  - Female
MH  - Horses/*blood/physiology
MH  - Leukocyte Count/veterinary
MH  - Male
MH  - Platelet Count/veterinary
MH  - Platelet-Derived Growth Factor/*metabolism/physiology
MH  - Platelet-Rich Plasma/*cytology/metabolism/physiology
MH  - Protein Isoforms
MH  - Sex Factors
MH  - Species Specificity
MH  - Transforming Growth Factor beta1/metabolism/physiology
PMC - PMC3577464
EDAT- 2013/02/14 06:00
MHDA- 2013/07/03 06:00
PMCR- 2013/02/12
CRDT- 2013/02/14 06:00
PHST- 2012/09/12 00:00 [received]
PHST- 2013/01/23 00:00 [accepted]
PHST- 2013/02/14 06:00 [entrez]
PHST- 2013/02/14 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
PHST- 2013/02/12 00:00 [pmc-release]
AID - 1746-6148-9-29 [pii]
AID - 10.1186/1746-6148-9-29 [doi]
PST - epublish
SO  - BMC Vet Res. 2013 Feb 12;9:29. doi: 10.1186/1746-6148-9-29.