PMID- 23402545
OWN - NLM
STAT- MEDLINE
DCOM- 20140916
LR  - 20211203
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 98
IP  - 1
DP  - 2014 Feb
TI  - Gene expression analysis of protein synthesis pathways in bovine mammary 
      epithelial cells purified from milk during lactation and short-term restricted 
      feeding.
PG  - 84-95
LID - 10.1111/jpn.12039 [doi]
AB  - The objective of the study was to investigate selected key regulatory pathways of 
      milk protein biosynthesis in primary bovine mammary epithelial cells (MECs) of 
      dairy cows during the first 155 days of lactation. In addition, cows were exposed 
      to feed restriction for a short period (FR) during different stages of lactation 
      (week 4 and 21 pp) to study adjustment processes of molecular protein 
      biosynthesis to metabolic challenge. Morning milk samples from twenty-four 
      Holstein-Friesian cows were collected throughout the experimental period (n = 10 
      per animal). MEC from raw milk were purified using an immunomagnetic separation 
      technique and used for real-time quantitative PCR analyses. As was seen in 
      transcript abundances of all major milk proteins, mRNA levels of E74-like factor 
      5 (ELF5), an enhancer of signal transducer and activator of transcription (STAT) 
      action, concomitantly decreased towards mid-lactation. Expression of ELF5 as well 
      as of all milk protein genes showed a similar increase during FR in early 
      lactation. Occasional changes in expression could be seen in other Janus kinase 
      (JAK)/STAT factors and in mammalian target of rapamycin (mTOR) pathway elements. 
      Amino acid transfer and glucose transporter and the beta-casein expression were also 
      partially affected. In conclusion, our findings suggest a pivotal role of the 
      transcription factor ELF5 in milk protein mRNA expression with complementary 
      JAK/STAT and mTOR signalling for the regulation of protein biosynthesis in the 
      bovine mammary gland.
CI  - (c) 2013 Blackwell Verlag GmbH.
FAU - Sigl, T
AU  - Sigl T
AD  - Physiology Weihenstephan, ZIEL, Technische Universitaet Muenchen, Freising, 
      Germany.
FAU - Meyer, H H D
AU  - Meyer HH
FAU - Wiedemann, S
AU  - Wiedemann S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130213
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (Amino Acids)
RN  - 0 (Glucose Transport Proteins, Facilitative)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT Transcription Factors)
RN  - EC 2.7.10.2 (Janus Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animal Feed/analysis
MH  - Animals
MH  - Biological Transport
MH  - Cattle
MH  - Diet/veterinary
MH  - Epithelial Cells/*metabolism
MH  - Female
MH  - Food Deprivation/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Glucose Transport Proteins, Facilitative/genetics/metabolism
MH  - Janus Kinases/genetics/metabolism
MH  - Lactation/*physiology
MH  - Mammary Glands, Animal/*cytology
MH  - Milk/chemistry/*cytology
MH  - RNA, Messenger/genetics/metabolism
MH  - STAT Transcription Factors/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
OTO - NOTNLM
OT  - JAK/STAT
OT  - cell signalling
OT  - gene expression
OT  - mTOR
OT  - primary mammary epithelial cells
OT  - transcription factor
EDAT- 2013/02/14 06:00
MHDA- 2014/09/17 06:00
CRDT- 2013/02/14 06:00
PHST- 2012/08/23 00:00 [received]
PHST- 2013/01/04 00:00 [accepted]
PHST- 2013/02/14 06:00 [entrez]
PHST- 2013/02/14 06:00 [pubmed]
PHST- 2014/09/17 06:00 [medline]
AID - 10.1111/jpn.12039 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2014 Feb;98(1):84-95. doi: 10.1111/jpn.12039. 
      Epub 2013 Feb 13.