PMID- 23402628
OWN - NLM
STAT- MEDLINE
DCOM- 20130816
LR  - 20181202
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 29
IP  - 9
DP  - 2013 Mar 5
TI  - Oxidation of tertiary amine-derivatized surfaces to control protein adhesion.
PG  - 2961-70
LID - 10.1021/la4003719 [doi]
AB  - Selective oxidation of omega-tertiary amine self-assembled thiol monolayers to 
      tertiary amine N-oxides is shown to transform the adhesion of model proteins 
      lysozyme and fibrinogen upon them. Efficient preparation of both secondary and 
      tertiary linker amides as judged by X-ray photoelectron spectroscopy (XPS) and 
      water droplet contact angle was achieved with an improved amide bond formation on 
      gold quartz crystal microbalance (QCM) sensors using 
      2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl hexafluorophosphate 
      methanaminium uronium (HATU). Oxidation with hydrogen peroxide was similarly 
      assessed, and adhesion of lysozyme and fibrinogen from phosphate buffered saline 
      was then assayed by QCM and imaged by AFM. Tertiary amine-functionalized sensors 
      adsorbed multilayers of aggregated lysozyme, whereas tertiary amine N-oxides and 
      triethylene glycol-terminated monolayers are consistent with small protein 
      aggregates. The surface containing a dimethylamine N-oxide headgroup and ethyl 
      secondary amide linker showed the largest difference in adsorption of both 
      proteins. Oxidation of tertiary amine decorated surfaces therefore holds the 
      potential for selective deposition of proteins and cells through masking and 
      other patterning techniques.
FAU - Dobrzanska, Dorota A
AU  - Dobrzanska DA
AD  - Department of Chemistry, and School of Life Sciences, University of Warwick, 
      Coventry CV4 7AL, United Kingdom.
FAU - Cooper, Amy L
AU  - Cooper AL
FAU - Dowson, Christopher G
AU  - Dowson CG
FAU - Evans, Stephen D
AU  - Evans SD
FAU - Fox, David J
AU  - Fox DJ
FAU - Johnson, Benjamin R
AU  - Johnson BR
FAU - Biggs, Caroline I
AU  - Biggs CI
FAU - Randev, Rajan K
AU  - Randev RK
FAU - Stec, Helena M
AU  - Stec HM
FAU - Taylor, Paul C
AU  - Taylor PC
FAU - Marsh, Andrew
AU  - Marsh A
LA  - eng
GR  - Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130222
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Adhesives)
RN  - 0 (Amides)
RN  - 0 (Amines)
RN  - 0 (Oxides)
RN  - 0 (Proteins)
RN  - 3P5SU53360 (triethylene glycol)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9001-32-5 (Fibrinogen)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Adhesives/*chemistry
MH  - Amides/chemistry
MH  - Amines/*chemistry
MH  - Animals
MH  - Fibrinogen/chemistry
MH  - Humans
MH  - Muramidase/chemistry
MH  - Oxidation-Reduction
MH  - Oxides/chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Proteins/*chemistry
MH  - Surface Properties
EDAT- 2013/02/14 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/02/14 06:00
PHST- 2013/02/14 06:00 [entrez]
PHST- 2013/02/14 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - 10.1021/la4003719 [doi]
PST - ppublish
SO  - Langmuir. 2013 Mar 5;29(9):2961-70. doi: 10.1021/la4003719. Epub 2013 Feb 22.