PMID- 23403103
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20220409
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 541
DP  - 2013 Apr 29
TI  - Mesenchymal stem cells from trabecular meshwork become photoreceptor-like cells 
      on amniotic membrane.
PG  - 43-8
LID - S0304-3940(13)00017-7 [pii]
LID - 10.1016/j.neulet.2012.12.055 [doi]
AB  - Stem cell therapy is a promising approach for treatment of degenerative retinal 
      disorders such as retinitis pigmentosa (RP) and age-related macular degeneration 
      (AMD). In this study, human mesenchymal stem cells (MSCs) were isolated from the 
      trabecular meshwork (TM), the major functional tissue of the anterior chamber 
      angle in the eye, were characterized and differentiated into photoreceptor cells 
      on amniotic membrane (AM). After isolation of trabecular meshwork and culture of 
      the stromal segment of this tissue, fibroblast-like cells (CD105(+), CD90(+), 
      CD44(+), CD166(+) cells) capable of differentiation toward mesenchymal and 
      photoreceptor lineages were obtained. The isolated cells were seeded on amniotic 
      membrane and were treated with induction medium. Immunocytochemistry and 
      quantitative real time RT-PCR (qPCR) were used to detect expression of 
      photoreceptor genes such as rhodopsin, recoverin, CRX, and peripherin; and the 
      bipolar cell marker protein kinase C alpha (PKC-alpha). As a result, 
      immunocytochemistry revealed that the differentiated TMMSCs expressed rhodopsin, 
      CRX and PKC proteins. qPCR showed the expression of rhodopsin (rod like 
      photoreceptor-specific marker), and CRX genes were significantly higher in TMMSCs 
      differentiated on AM than those differentiated on tissue culture polystyrene 
      (TCPS). In conclusion, our findings suggested that a combination of TMMSCs (as a 
      new source) and basement membrane support from AM might be a suitable source of 
      cells for subretinal transplantation in regenerative therapy for retinal 
      disorders such as AMD and RP.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Nadri, Samad
AU  - Nadri S
AD  - Medical Physics and Biomedical Engineering Department, Faculty of Medicine, 
      Shahid Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Yazdani, Shahin
AU  - Yazdani S
FAU - Arefian, Ehsan
AU  - Arefian E
FAU - Gohari, Zahra
AU  - Gohari Z
FAU - Eslaminejad, Mohamadreza Baghaban
AU  - Eslaminejad MB
FAU - Kazemi, Bahram
AU  - Kazemi B
FAU - Soleimani, Masoud
AU  - Soleimani M
LA  - eng
PT  - Journal Article
DEP - 20130208
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Amnion/*cytology
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Photoreceptor Cells, Vertebrate/*cytology/metabolism
MH  - Trabecular Meshwork/*cytology
EDAT- 2013/02/14 06:00
MHDA- 2013/09/04 06:00
CRDT- 2013/02/14 06:00
PHST- 2012/09/08 00:00 [received]
PHST- 2012/12/28 00:00 [revised]
PHST- 2012/12/29 00:00 [accepted]
PHST- 2013/02/14 06:00 [entrez]
PHST- 2013/02/14 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
AID - S0304-3940(13)00017-7 [pii]
AID - 10.1016/j.neulet.2012.12.055 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 Apr 29;541:43-8. doi: 10.1016/j.neulet.2012.12.055. Epub 2013 
      Feb 8.