PMID- 23405277
OWN - NLM
STAT- MEDLINE
DCOM- 20130819
LR  - 20220223
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 3
DP  - 2013
TI  - Identification of cardiolipin binding sites on cytochrome c oxidase at the 
      entrance of proton channels.
PG  - 1263
LID - 10.1038/srep01263 [doi]
LID - 1263
AB  - The respiratory chain or oxidative phosphorylation system (OxPhos) generates most 
      of the chemical energy (ATP) used by our cells. The cytochrome c oxidase (CcO) is 
      one of three protein complexes of OxPhos building up a proton gradient across the 
      inner mitochondrial membrane, which is ultimately used by the ATP synthase to 
      produce ATP. We present molecular dynamic simulations of CcO in a mimic of the 
      mitochondrial membrane, and identify precise binding sites of cardiolipin (CL, 
      signature phospholipid of mitochondria) on the protein surface. Two of these CL 
      binding sites reveal pathways linking CLs to the entrance of the D and H proton 
      channels across CcO. CLs being able to carry protons our results strongly support 
      an involvement of CLs in the proton delivery machinery to CcO. The ubiquitous 
      nature of CL interactions with the components of the OxPhos suggests that this 
      delivery mechanism might extend to the other respiratory complexes.
FAU - Arnarez, C
AU  - Arnarez C
AD  - Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute 
      for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG Groningen, 
      The Netherlands.
FAU - Marrink, S J
AU  - Marrink SJ
FAU - Periole, X
AU  - Periole X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130212
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cardiolipins)
RN  - 0 (Lipids)
RN  - 0 (Protons)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
EIN - Sci Rep. 2013;3:1343
MH  - Animals
MH  - Binding Sites
MH  - Cardiolipins/chemistry/*metabolism
MH  - Cattle
MH  - Crystallography, X-Ray
MH  - Electron Transport Complex IV/chemistry/*metabolism
MH  - Hydrogen Bonding
MH  - Lipids/chemistry
MH  - Molecular Docking Simulation
MH  - Protein Structure, Tertiary
MH  - Protons
PMC - PMC3570132
EDAT- 2013/02/14 06:00
MHDA- 2013/08/21 06:00
PMCR- 2013/02/12
CRDT- 2013/02/14 06:00
PHST- 2012/11/20 00:00 [received]
PHST- 2013/01/07 00:00 [accepted]
PHST- 2013/02/14 06:00 [entrez]
PHST- 2013/02/14 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
PHST- 2013/02/12 00:00 [pmc-release]
AID - srep01263 [pii]
AID - 10.1038/srep01263 [doi]
PST - ppublish
SO  - Sci Rep. 2013;3:1263. doi: 10.1038/srep01263. Epub 2013 Feb 12.