PMID- 23405950
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20191210
IS  - 2159-3345 (Electronic)
IS  - 2159-3337 (Linking)
VI  - 23
IP  - 2
DP  - 2013 Apr
TI  - Evaluation of biodegradable peptide carriers for light-directed targeting.
PG  - 131-9
LID - 10.1089/nat.2012.0403 [doi]
AB  - A promising strategy for increased intracellular delivery of nucleic acids with 
      the benefit for targeting is photochemical internalization (PCI). PCI relies on 
      the use of a photosensitizing compound that photochemically destroys membranes in 
      the endocytic pathway after illumination, resulting in cytosolic transfer of 
      endosomal content. PCI technology combined with biodegradable polyamino acid 
      carriers and nucleic acids delivers effective targeting and improved biosafety. 
      In an in vitro model system, we have evaluated various poly-l-lysine (PLL), 
      poly-l-histidine (PLH), and poly-l-arginine (PLA) formulations for light-directed 
      small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. 
      We find that PLA formulations are suitable as siRNA and mRNA carriers in a 
      strictly light-directed manner.
FAU - Jorgensen, Jens Andreas Lindin
AU  - Jorgensen JA
AD  - Department of Tumor Biology, Institute for Cancer Research , The Norwegian Radium 
      Hospital, Oslo, Norway .
FAU - Longva, Ane Sager
AU  - Longva AS
FAU - Hovig, Eivind
AU  - Hovig E
FAU - Boe, Sigurd Leinaes
AU  - Boe SL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130213
PL  - United States
TA  - Nucleic Acid Ther
JT  - Nucleic acid therapeutics
JID - 101562758
RN  - 0 (Nucleic Acids)
RN  - 0 (Peptides)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (RNA, Small Interfering)
RN  - 94ZLA3W45F (Arginine)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Arginine/administration & dosage/chemistry
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Endosomes/chemistry/metabolism
MH  - Genetic Therapy
MH  - Humans
MH  - Light
MH  - Lysine/administration & dosage/chemistry
MH  - *Melanoma/drug therapy/genetics
MH  - *Molecular Targeted Therapy
MH  - Nucleic Acids/administration & dosage/chemistry
MH  - *Peptides/administration & dosage/chemistry
MH  - Photosensitizing Agents/administration & dosage/chemistry
MH  - RNA, Small Interfering/*administration & dosage/chemistry
MH  - Transfection
EDAT- 2013/02/15 06:00
MHDA- 2013/09/24 06:00
CRDT- 2013/02/15 06:00
PHST- 2013/02/15 06:00 [entrez]
PHST- 2013/02/15 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
AID - 10.1089/nat.2012.0403 [doi]
PST - ppublish
SO  - Nucleic Acid Ther. 2013 Apr;23(2):131-9. doi: 10.1089/nat.2012.0403. Epub 2013 
      Feb 13.