PMID- 23408504
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20211021
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 85
IP  - 5
DP  - 2013 May
TI  - Frequent detection of cytomegalovirus (CMV) DNA in the lower respiratory tract in 
      CMV-seropositive pediatric patients with underlying chronic bronchopulmonary 
      diseases lacking canonical immunosuppression.
PG  - 888-92
LID - 10.1002/jmv.23499 [doi]
AB  - Cytomegalovirus (CMV) may be a relevant cause of morbidity in patients displaying 
      various inflammatory diseases. In this study, it was investigated whether CMV DNA 
      is detected in the lower respiratory tract and the systemic compartment in 
      pediatric patients with chronic or recurrent bronchopulmonary diseases. A total 
      of 42 lower respiratory tract specimens and 11 paired plasma samples from 42 
      patients were analyzed for the presence of CMV DNA by real-time PCR. The 
      respiratory specimens were also screened for the presence of respiratory viruses 
      and human herpesvirus 6 (HHV-6) and 7 (HHV-7) by PCR methods. Quantitative 
      bacterial and fungal cultures were performed. IL-6 levels in the respiratory 
      specimens were quantified using ELISA. CMV DNA was detected either in the lower 
      respiratory airways, in plasma, or both in 54.5% of CMV-seropositive patients. 
      The levels of IL-6 were significantly higher in these patients than in those with 
      no detectable levels of CMV DNA. HHV-6 and HHV-7 DNA were detected in three and 
      one patients, respectively. Respiratory viruses were detected in 13 of the 42 
      patients. Significant growth of one or more bacterial species was observed in 17 
      patients. No significant association was found between the presence of CMV DNA 
      and the detection of other microorganisms. The data indicated that the presence 
      of CMV DNA in the lower respiratory tract is a frequent finding in children with 
      chronic or recurrent bronchopulmonary diseases. Further, prospective 
      observational studies are needed to assess the impact of this phenomenon, if any, 
      on the clinical course of these patients.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Escribano, Amparo
AU  - Escribano A
AD  - Pediatric Service, Hospital Clinico Universitario, Valencia, Spain.
FAU - Chilet, Marifina
AU  - Chilet M
FAU - Clari, Maria Angeles
AU  - Clari MA
FAU - Lucas, Raquel
AU  - Lucas R
FAU - Costa, Elisa
AU  - Costa E
FAU - Bravo, Dayana
AU  - Bravo D
FAU - Munoz-Cobo, Beatriz
AU  - Munoz-Cobo B
FAU - Borras, Rafael
AU  - Borras R
FAU - Navarro, David
AU  - Navarro D
LA  - eng
PT  - Journal Article
DEP - 20130213
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Adolescent
MH  - Antibodies, Viral/*blood
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Cytomegalovirus/*isolation & purification
MH  - DNA, Viral/*blood/*isolation & purification
MH  - Female
MH  - Humans
MH  - Infant
MH  - Interleukin-6/blood
MH  - Lung Diseases/*epidemiology/virology
MH  - Male
MH  - Plasma/*virology
MH  - Real-Time Polymerase Chain Reaction
MH  - Recurrence
MH  - Respiratory System/*virology
PMC - PMC7166780
EDAT- 2013/02/15 06:00
MHDA- 2013/09/05 06:00
PMCR- 2013/02/13
CRDT- 2013/02/15 06:00
PHST- 2012/11/19 00:00 [accepted]
PHST- 2013/02/15 06:00 [entrez]
PHST- 2013/02/15 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
PHST- 2013/02/13 00:00 [pmc-release]
AID - JMV23499 [pii]
AID - 10.1002/jmv.23499 [doi]
PST - ppublish
SO  - J Med Virol. 2013 May;85(5):888-92. doi: 10.1002/jmv.23499. Epub 2013 Feb 13.