PMID- 23408706
OWN - NLM
STAT- MEDLINE
DCOM- 20141203
LR  - 20171116
IS  - 1930-739X (Electronic)
IS  - 1930-7381 (Linking)
VI  - 21
IP  - 11
DP  - 2013 Nov
TI  - Markers of macrophage infiltration and measures of lipolysis in human abdominal 
      adipose tissues.
PG  - 2342-9
LID - 10.1002/oby.20341 [doi]
AB  - OBJECTIVE: We tested the hypothesis that high lipolytic responsiveness is related 
      to increased expression of ATM genes in human adipose tissues. DESIGN AND 
      METHODS: Omental (OM) and subcutaneous (SC) fat samples were obtained surgically 
      in 46 women (age: 47.2 +/- 4.7 years, BMI: 26.9 +/- 5.2 kg/m(2) ). Body composition 
      and fat distribution were measured using dual energy X-ray absorptiometry and 
      computed tomography. Lipolysis was measured by glycerol release in mature 
      adipocytes isolated by collagenase digestion under basal-, isoproterenol (10(-5) 
      M)-, and forskolin (10(-5) M)-stimulated conditions. Quantification of macrophage 
      gene mRNA expression (CD11b, CD11c, and CD68) in whole adipose tissue was 
      performed using real-time RT-PCR. RESULTS: SC CD68 mRNA abundance was positively 
      associated with isoproterenol-stimulated lipolysis (r = 0.36, P < 0.05). This 
      association remained significant after adjustment for total body fat mass (r = 
      0.34, P </= 0.05). In the OM depot, CD11b mRNA abundance was positively associated 
      with isoproterenol-stimulated lipolysis (r = 0.42, P </= 0.005). This association 
      remained significant after adjustment for total body fat mass (r = 0.41, P </= 
      0.01). In subgroup analyses, high lipolytic rates in SC adipocytes were related 
      to increased whole tissue expression of CD68 and CD11b in this compartment, 
      independent of adiposity and fat cell size (P </= 0.001 and P </= 0.05). High 
      lipolytic rates in OM adipocytes were related to increased whole tissue OM 
      expression of CD11b, independent of adiposity and fat cell size (P </= 0.05). 
      CONCLUSIONS: High adipocyte lipolytic responsiveness is related to increased 
      expression of ATM markers in the corresponding compartment, independent of 
      adiposity and fat cell size.
CI  - Copyright (c) 2013 The Obesity Society.
FAU - Michaud, Andreanne
AU  - Michaud A
AD  - Endocrinology and Genomics, Laval University Medical Center, 2705 Laurier Blvd. 
      (T3-67), Quebec G1V 4G2, Canada; Department of Nutrition, Laval University, 
      Pavillon Paul Comtois, Quebec G1K 7P4, Canada.
FAU - Pelletier, Melissa
AU  - Pelletier M
FAU - Noel, Suzanne
AU  - Noel S
FAU - Bouchard, Celine
AU  - Bouchard C
FAU - Tchernof, Andre
AU  - Tchernof A
LA  - eng
GR  - MOP-64182/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130525
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Biomarkers)
RN  - 0 (CD11 Antigens)
RN  - 0 (CD68 antigen, human)
RN  - EC 2.7.11.1 (ATM protein, human)
RN  - EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins)
RN  - L628TT009W (Isoproterenol)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Abdominal Fat/drug effects/*metabolism
MH  - Adult
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Ataxia Telangiectasia Mutated Proteins/genetics/metabolism
MH  - Biomarkers/*analysis/metabolism
MH  - CD11 Antigens/genetics/metabolism
MH  - Cell Movement/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Glycerol/metabolism
MH  - Humans
MH  - Isoproterenol/pharmacology
MH  - Lipolysis/drug effects/*genetics
MH  - Macrophages/*physiology
MH  - Middle Aged
EDAT- 2013/02/15 06:00
MHDA- 2014/12/15 06:00
CRDT- 2013/02/15 06:00
PHST- 2012/04/19 00:00 [received]
PHST- 2012/12/04 00:00 [accepted]
PHST- 2013/02/15 06:00 [entrez]
PHST- 2013/02/15 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1002/oby.20341 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2013 Nov;21(11):2342-9. doi: 10.1002/oby.20341. Epub 
      2013 May 25.