PMID- 23408758
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20221207
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 121
IP  - 8
DP  - 2013 Aug
TI  - Prediction of outcome in patients with low-grade squamous intraepithelial lesions 
      by fluorescence in situ hybridization analysis of human papillomavirus, TERC, and 
      MYC.
PG  - 423-31
LID - 10.1002/cncy.21280 [doi]
AB  - BACKGROUND: Cytology is an excellent method with which to diagnose preinvasive 
      lesions of the uterine cervix, but it suffers from limited specificity for 
      clinically significant lesions. Supplementary methods might predict the natural 
      course of the detected lesions. The objective of the current study was to test 
      whether a multicolor fluorescence in situ hybridization (FISH) assay might help 
      to stratify abnormal results of Papanicolaou tests. METHODS: A total of 219 
      liquid-based cytology specimens of low-grade squamous intraepithelial lesions 
      (LSIL), 49 atypical squamous cells of undetermined significance (ASCUS) 
      specimens, 52 high-grade squamous intraepithelial lesion (HSIL) specimens, and 50 
      normal samples were assessed by FISH with probes for the human papillomavirus 
      (HPV), MYC, and telomerase RNA component (TERC). Subtyping of HPV by polymerase 
      chain reaction (PCR) was performed in a subset of cases (n=206). RESULTS: There 
      was a significant correlation found between HPV detection by FISH and PCR 
      (P<.0001). In patients with LSILs, the presence of HPV detected by FISH was 
      significantly associated with disease progression (P<.0001). An increased MYC 
      and/or TERC gene copy number (>2 signals in>10% of cells) prevailed in 43% of 
      ASCUS specimens and was more frequent in HSIL (85%) than in LSIL (33%) (HSIL vs 
      LSIL: P<.0001). Increased TERC gene copy number was significantly correlated with 
      progression of LSIL (P<.01; odds ratio, 7.44; area under the receiver operating 
      characteristic curve, 0.73; positive predictive value, 0.30; negative predictive 
      value, 0.94) CONCLUSIONS: The detection of HPV by FISH analysis is feasible in 
      liquid-based cytology and is significantly correlated with HPV analysis by PCR. 
      The analysis of TERC gene copy number may be useful for risk stratification in 
      patients with LSIL.
CI  - Copyright (c) 2013 American Cancer Society.
FAU - Obermann, Ellen C
AU  - Obermann EC
AD  - Institute of Pathology, University Hospital Basel, Basel, Switzerland. 
      Ellen.Obermann@usb.ch
FAU - Savic Prince, Spasenija
AU  - Savic Prince S
FAU - Barascud, Audrey
AU  - Barascud A
FAU - Grilli, Bruno
AU  - Grilli B
FAU - Herzog, Michelle
AU  - Herzog M
FAU - Kaup, Daniela
AU  - Kaup D
FAU - Cathomas, Gieri
AU  - Cathomas G
FAU - Frey Tirri, Brigitte
AU  - Frey Tirri B
FAU - Zlobec, Inti
AU  - Zlobec I
FAU - Wight, Edward
AU  - Wight E
FAU - Bubendorf, Lukas
AU  - Bubendorf L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130213
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Area Under Curve
MH  - Carcinoma, Squamous Cell/*diagnosis/virology
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Papillomaviridae
MH  - Papillomavirus Infections/complications/diagnosis/virology
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-myc/*analysis/genetics
MH  - RNA/*analysis/genetics
MH  - ROC Curve
MH  - Telomerase/*analysis/genetics
MH  - Uterine Cervical Neoplasms/*diagnosis/genetics/virology
MH  - Uterine Cervical Dysplasia/*diagnosis/genetics/virology
OTO - NOTNLM
OT  - MYC
OT  - cervical cytology
OT  - fluorescence in situ hybridization
OT  - human papillomavirus
OT  - squamous intraepithelial lesion
OT  - telomerase RNA component (TERC)
EDAT- 2013/02/15 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/02/15 06:00
PHST- 2012/12/10 00:00 [received]
PHST- 2012/12/27 00:00 [revised]
PHST- 2013/01/02 00:00 [accepted]
PHST- 2013/02/15 06:00 [entrez]
PHST- 2013/02/15 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
AID - 10.1002/cncy.21280 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2013 Aug;121(8):423-31. doi: 10.1002/cncy.21280. Epub 2013 Feb 
      13.