PMID- 23409932
OWN - NLM
STAT- MEDLINE
DCOM- 20130812
LR  - 20181202
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 26
IP  - 1
DP  - 2013 Feb
TI  - Immunization with DNA vaccines containing porcine reproductive and respiratory 
      syndrome virus open reading frames 5, 6, and 7 may be related to the exacerbation 
      of clinical disease after an experimental challenge.
PG  - 93-101
LID - 10.1089/vim.2012.0041 [doi]
AB  - Pigs were immunized with DNA plasmids containing different open reading frames 
      (ORFs) of a porcine reproductive and respiratory syndrome virus (PRRSV) genotype 
      I strain. One group was injected with three inoculations of ORF7, a second group 
      was immunized with three inoculations of plasmids containing ORF5 and ORF6, and a 
      third group was kept as controls. Later, +21 days after the last inoculation, 
      animals were challenged with the homologous strain. After the challenge, 
      PRRSV-specific interferon (IFN)-gamma-secreting cells and anti-PRRSV IgG antibodies 
      developed faster in DNA vaccinated pigs (p<0.05). However, DNA-immunized pigs 
      showed an exacerbation of the disease compared to the unvaccinated challenged 
      pigs. The data suggest that previous immunization with DNA vaccines against 
      glycoprotein 5 and/or matrix protein of PRRSV, as well as nucleoprotein but to a 
      lesser degree, could result in an exacerbation of the clinical course in terms of 
      fever upon challenge.
FAU - Diaz, Ivan
AU  - Diaz I
AD  - Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autonoma de 
      Barcelona, Bellaterra, Barcelona, Spain. ivan.diaz@cresa.uab.cat
FAU - Ganges, Llilianne
AU  - Ganges L
FAU - Galindo-Cardiel, Ivan
AU  - Galindo-Cardiel I
FAU - Tarradas, Joan
AU  - Tarradas J
FAU - Alvarez, Belen
AU  - Alvarez B
FAU - Lorca-Oro, Cristina
AU  - Lorca-Oro C
FAU - Pujols, Joan
AU  - Pujols J
FAU - Gimeno, Mariona
AU  - Gimeno M
FAU - Darwich, Laila
AU  - Darwich L
FAU - Domingo, Mariano
AU  - Domingo M
FAU - Dominguez, Javier
AU  - Dominguez J
FAU - Mateu, Enric
AU  - Mateu E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, DNA)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/genetics/*immunology
MH  - Immunization/*methods
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/*pathology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Severity of Illness Index
MH  - Swine
MH  - Vaccines, DNA/administration & dosage/genetics/*immunology
EDAT- 2013/02/16 06:00
MHDA- 2013/08/13 06:00
CRDT- 2013/02/16 06:00
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/13 06:00 [medline]
AID - 10.1089/vim.2012.0041 [doi]
PST - ppublish
SO  - Viral Immunol. 2013 Feb;26(1):93-101. doi: 10.1089/vim.2012.0041.