PMID- 23410158
OWN - NLM
STAT- MEDLINE
DCOM- 20130813
LR  - 20211021
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 14
DP  - 2013 Feb 14
TI  - Systematic and quantitative mRNA expression analysis of TRP channel genes at the 
      single trigeminal and dorsal root ganglion level in mouse.
PG  - 21
LID - 10.1186/1471-2202-14-21 [doi]
AB  - BACKGROUND: Somatosensory nerve fibres arising from cell bodies within the 
      trigeminal ganglia (TG) in the head and from a string of dorsal root ganglia 
      (DRG) located lateral to the spinal cord convey endogenous and environmental 
      stimuli to the central nervous system. Although several members of the transient 
      receptor potential (TRP) superfamily of cation channels have been implicated in 
      somatosensation, the expression levels of TRP channel genes in the individual 
      sensory ganglia have never been systematically studied. RESULTS: Here, we used 
      quantitative real-time PCR to analyse and compare mRNA expression of all TRP 
      channels in TG and individual DRGs from 27 anatomically defined segments of the 
      spinal cord of the mouse. At the mRNA level, 17 of the 28 TRP channel genes, 
      TRPA1, TRPC1, TRPC3, TRPC4, TRPC5, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, 
      TRPM8, TRPV1, TRPV2, TRPV4, TRPML1 and TRPP2, were detectable in every tested 
      ganglion. Notably, four TRP channels, TRPC4, TRPM4, TRPM8 and TRPV1, showed 
      statistically significant variation in mRNA levels between DRGs from different 
      segments, suggesting ganglion-specific regulation of TRP channel gene expression. 
      These ganglion-to-ganglion differences in TRP channel transcript levels may 
      contribute to the variability in sensory responses in functional studies. 
      CONCLUSIONS: We developed, compared and refined techniques to quantitatively 
      analyse the relative mRNA expression of all TRP channel genes at the single 
      ganglion level. This study also provides for the first time a comparative mRNA 
      distribution profile in TG and DRG along the entire vertebral column for the 
      mammalian TRP channel family.
FAU - Vandewauw, Ine
AU  - Vandewauw I
AD  - Laboratory of Ion Channel Research, Department of Cellular and Molecular Medicine 
      and TRP Research Platform Leuven, KU Leuven, Leuven, Belgium.
FAU - Owsianik, Grzegorz
AU  - Owsianik G
FAU - Voets, Thomas
AU  - Voets T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130214
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 0 (RNA, Messenger)
RN  - 0 (Transient Receptor Potential Channels)
SB  - IM
MH  - Animals
MH  - Ganglia, Spinal/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/*metabolism
MH  - Transient Receptor Potential Channels/*classification/*genetics/metabolism
MH  - Trigeminal Ganglion/*metabolism
PMC - PMC3576292
EDAT- 2013/02/16 06:00
MHDA- 2013/08/14 06:00
PMCR- 2013/02/14
CRDT- 2013/02/16 06:00
PHST- 2012/09/26 00:00 [received]
PHST- 2013/01/31 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/14 06:00 [medline]
PHST- 2013/02/14 00:00 [pmc-release]
AID - 1471-2202-14-21 [pii]
AID - 10.1186/1471-2202-14-21 [doi]
PST - epublish
SO  - BMC Neurosci. 2013 Feb 14;14:21. doi: 10.1186/1471-2202-14-21.