PMID- 23411211
OWN - NLM
STAT- MEDLINE
DCOM- 20130816
LR  - 20211203
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 138
IP  - 2-3
DP  - 2013 Jun 1
TI  - Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and 
      S phase cell cycle arrest in HepG2 cells.
PG  - 1034-41
LID - S0308-8146(12)01846-8 [pii]
LID - 10.1016/j.foodchem.2012.11.102 [doi]
AB  - This study investigates the anticancer properties of cannabisin B, purified from 
      hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that 
      cannabisin B significantly inhibited cell proliferation by inducing autophagic 
      cell death rather than typical apoptosis. Cell viability transiently increased 
      upon the addition of a low concentration of cannabisin B but decreased upon the 
      addition of high concentrations. Cannabisin B-induced changes in cell viability 
      were completely inhibited by pre-treatment with 3-methyladenine (3-MA), 
      indicating that the induction of autophagy by cannabisin B caused cell death. 
      Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent 
      manner. Moreover, cannabisin B was found to inhibit survival signaling by 
      blocking the activation of AKT and down-stream targets of the mammalian target of 
      rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable 
      antiproliferative activity and that it may be utilised as a promising 
      chemopreventive agent against hepatoblastoma disease.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Chen, Tianpeng
AU  - Chen T
AD  - College of Engineering, Peking University, Beijing 100871, China.
FAU - Hao, Jianxiong
AU  - Hao J
FAU - He, Jinfeng
AU  - He J
FAU - Zhang, Jianchun
AU  - Zhang J
FAU - Li, Yingcong
AU  - Li Y
FAU - Liu, Rui
AU  - Liu R
FAU - Li, Lite
AU  - Li L
LA  - eng
PT  - Journal Article
DEP - 20121205
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Plant Extracts)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Autophagy/*drug effects
MH  - Cannabis/*chemistry
MH  - Down-Regulation/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Plant Extracts/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/genetics/*metabolism
MH  - S Phase Cell Cycle Checkpoints/*drug effects
MH  - Seeds/*chemistry
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2013/02/16 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/02/16 06:00
PHST- 2012/07/06 00:00 [received]
PHST- 2012/11/09 00:00 [revised]
PHST- 2012/11/21 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0308-8146(12)01846-8 [pii]
AID - 10.1016/j.foodchem.2012.11.102 [doi]
PST - ppublish
SO  - Food Chem. 2013 Jun 1;138(2-3):1034-41. doi: 10.1016/j.foodchem.2012.11.102. Epub 
      2012 Dec 5.