PMID- 23411288
OWN - NLM
STAT- MEDLINE
DCOM- 20130816
LR  - 20191210
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 138
IP  - 2-3
DP  - 2013 Jun 1
TI  - A novel colloidal gold-based lateral flow immunoassay for rapid simultaneous 
      detection of cyromazine and melamine in foods of animal origin.
PG  - 1610-5
LID - S0308-8146(12)01809-2 [pii]
LID - 10.1016/j.foodchem.2012.11.077 [doi]
AB  - A rapid and sensitive lateral flow immunoassay (LFIA) based on competitive format 
      was developed and validated for simultaneous detection of cyromazine (CA) and 
      melamine (MA) in foods of animal origin. With this method, the cut-off value for 
      the two test lines were achieved at 25 ng/g, which was lower than the maximum 
      residue levels (MRLs) established for CA and MA. At three fortified levels (50, 
      100, and 150 ng/g), the recoveries for CA and MA ranged from 73.9% to 104.2% with 
      the relative standard deviation (RSD) less than 11.9%, based on within day and 
      interday analysis. The lower detection limit for CA and MA in matrix sample were 
      0.22 ng/ml and 0.26 ng/ml, respectively, which were lower than those of published 
      literatures. A parallel analysis of CA and MA in real samples conducted by HPLC 
      showed comparable results to those obtained from LFIA. The results of LFIA were 
      in good agreement with those of high performance liquid chromatography (HPLC) in 
      the analysis of CA and MA in foods of animal origin, demonstrating the practical 
      applicability of the developed assay in real samples. Overall, to our knowledge, 
      this is the first report of quantitative or semi-quantitative simultaneous 
      detection for CA and MA by immunochromatographic assay.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Le, Tao
AU  - Le T
AD  - College of Life Science, Chongqing Normal University, Chongqing 401331, PR China. 
      hnxylt@163.com
FAU - Yan, Peifeng
AU  - Yan P
FAU - Xu, Jian
AU  - Xu J
FAU - Hao, Youjing
AU  - Hao Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121129
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Gold Colloid)
RN  - 0 (Pesticides)
RN  - 0 (Triazines)
RN  - CA49Y29RA9 (cyromazine)
RN  - N3GP2YSD88 (melamine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chickens
MH  - Food Contamination/*analysis
MH  - Gold Colloid/chemistry
MH  - Immunoassay/instrumentation/*methods
MH  - Limit of Detection
MH  - Liver/chemistry
MH  - Meat/*analysis
MH  - Milk/*chemistry
MH  - Pesticides/*analysis
MH  - Sheep
MH  - Swine
MH  - Triazines/*analysis
EDAT- 2013/02/16 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/02/16 06:00
PHST- 2011/05/29 00:00 [received]
PHST- 2012/04/11 00:00 [revised]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0308-8146(12)01809-2 [pii]
AID - 10.1016/j.foodchem.2012.11.077 [doi]
PST - ppublish
SO  - Food Chem. 2013 Jun 1;138(2-3):1610-5. doi: 10.1016/j.foodchem.2012.11.077. Epub 
      2012 Nov 29.