PMID- 23411402
OWN - NLM
STAT- MEDLINE
DCOM- 20140130
LR  - 20130528
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 58
IP  - 7
DP  - 2013 Jul
TI  - Dental pulp and gingivomucosa in rats are innervated by two morphologically and 
      neurochemically different populations of nociceptors.
PG  - 788-95
LID - S0003-9969(13)00011-3 [pii]
LID - 10.1016/j.archoralbio.2013.01.006 [doi]
AB  - OBJECTIVES: Difference in phenotypes of sensory neurons innervating dental pulp 
      or gingivomucosa may be responsible for intense pain sensations in pulpitis in 
      contrast to relatively painless chronic periodontitis. Therefore, we classified 
      these neurons according to their size and two neurochemical characteristics of 
      nociceptors, their TrkA expression and isolectin IB4 binding. DESIGN: In rats 
      (n=6) fluorescent tracers Fluorogold and TrueBlue were simultaneously applied 
      into the standard-sized tooth cavity and nearby gingival sulcus, respectively. 
      After the fluorescence on paraffin trigeminal ganglia (TG) sections was 
      identified and photographed, immunohistochemistry for TrkA expression and IB4 
      binding was performed on the same sections. RESULTS: The average sizes of TG 
      neurons projecting to the gingivomucosa and dental pulp were 894+/-441mum(2) and 
      1012+/-381mum(2), respectively. The proportions of small-sized gingival and pulpal 
      neurons were 14% and 5%, respectively (p<0.05). The proportions of TrkA-positive 
      neurons among all gingival or pulpal neurons were 76% and 86%, respectively 
      (p<0.05). Among all gingival or pulpal neurons the proportions of IB4-positive 
      neurons were 46% and 3% (p<0.001), respectively, and the majority of them were 
      small-medium sized. CONCLUSIONS: Dental pulp and gingivomucosa are richly 
      innervated by nociceptive TrkA-expressing neurons. However, while great majority 
      of pulpal neurons are larger NGF-dependent A-fibre nociceptors without affinity 
      to bind IB4, almost half of the gingival neurons are smaller IB4 binding C-fibre 
      nociceptors. The difference in phenotype of sensory neurons might partially 
      explain the different sensitivity of both tissues during normal and pathological 
      conditions.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Kovacic, Uros
AU  - Kovacic U
AD  - Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, 
      Zaloska 2, 1000 Ljubljana, Slovenia.
FAU - Tesovnik, Blaz
AU  - Tesovnik B
FAU - Molnar, Nikola
AU  - Molnar N
FAU - Cor, Andrej
AU  - Cor A
FAU - Skaleric, Uros
AU  - Skaleric U
FAU - Gaspersic, Rok
AU  - Gaspersic R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130211
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Griffonia simplicifolia lectins)
RN  - 0 (Plant Lectins)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
MH  - Animals
MH  - Dental Pulp/*innervation/metabolism
MH  - Female
MH  - Gingiva/*innervation/metabolism
MH  - Immunohistochemistry
MH  - Mouth Mucosa/*innervation
MH  - Nociceptors/*metabolism
MH  - Plant Lectins/immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkA/*metabolism
MH  - Trigeminal Ganglion/*cytology/metabolism
EDAT- 2013/02/16 06:00
MHDA- 2014/01/31 06:00
CRDT- 2013/02/16 06:00
PHST- 2012/10/01 00:00 [received]
PHST- 2013/01/14 00:00 [revised]
PHST- 2013/01/17 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2014/01/31 06:00 [medline]
AID - S0003-9969(13)00011-3 [pii]
AID - 10.1016/j.archoralbio.2013.01.006 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2013 Jul;58(7):788-95. doi: 10.1016/j.archoralbio.2013.01.006. 
      Epub 2013 Feb 11.