PMID- 23412420
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20211021
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 9
IP  - 2
DP  - 2013
TI  - A novel brain neurovascular unit model with neurons, astrocytes and microvascular 
      endothelial cells of rat.
PG  - 174-89
LID - 10.7150/ijbs.5115 [doi]
AB  - A novel triple cell neurovascular unit (NVU) model co-culturing with neurons, 
      brain microvascular endothelial cells (BMECs) and astrocytes was established in 
      this study for investigating the cerebral diseases and screening the candidates 
      of therapeutic drug. We have first performed the cell identification and 
      morphological characterization, analyzed the specific protein expression and 
      determined the blood-brain barrier (BBB) function of the co-culture model under 
      normal condition. Then, we further determined the BBB function, inflammation, 
      cell injury and the variation of neuroprotective factor in this model after 
      anoxia-reoxygenation. The results suggest that this model exhibited a better BBB 
      function and significantly increased expression of P-glycoprotein (Pg-P) and ZO-1 
      compared with BMECs only or co-culture with astrocytes or neurons. After 
      anoxia-reoxygenation, the pathological changes of this model were basically 
      resemblance to the pathological changes of brain cells and BBB in vivo. And 
      nimodipine, an antagonist of calcium, could reverse those changes as well. 
      According to our observations, we deduce that this triple cell co-culture model 
      exhibits the basic structure, function and cell-cell interaction of NVU, which 
      may offer a more proper in vitro system of NVU for the further investigation of 
      cerebral diseases and drug screening.
FAU - Xue, Qiang
AU  - Xue Q
AD  - College of Pharmaceutical Sciences & College of Chinese Medicine, Southwest 
      University, Chongqing 400715, China.
FAU - Liu, Yang
AU  - Liu Y
FAU - Qi, Hongyi
AU  - Qi H
FAU - Ma, Qiang
AU  - Ma Q
FAU - Xu, Ling
AU  - Xu L
FAU - Chen, Weihai
AU  - Chen W
FAU - Chen, Gang
AU  - Chen G
FAU - Xu, Xiaoyu
AU  - Xu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130207
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Astrocytes/*cytology
MH  - Blood-Brain Barrier/*ultrastructure
MH  - Blotting, Western
MH  - Brain/*cytology
MH  - Cell Culture Techniques
MH  - Endothelial Cells/*cytology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescein
MH  - Horseradish Peroxidase
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Transmission
MH  - Microvessels/*cytology
MH  - *Models, Neurological
MH  - Neurons/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC3572400
OTO - NOTNLM
OT  - anoxia-reoxygenation
OT  - astrocytes
OT  - brain microvascular endothelial cells
OT  - neurons.
OT  - neurovascular unit
OT  - triple cell co-culture model
COIS- Conflict of Interest: The authors confirm that there are no conflicts of 
      interest.
EDAT- 2013/02/16 06:00
MHDA- 2013/08/06 06:00
PMCR- 2013/01/01
CRDT- 2013/02/16 06:00
PHST- 2012/08/27 00:00 [received]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - ijbsv09p0174 [pii]
AID - 10.7150/ijbs.5115 [doi]
PST - ppublish
SO  - Int J Biol Sci. 2013;9(2):174-89. doi: 10.7150/ijbs.5115. Epub 2013 Feb 7.