PMID- 23412837
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20211021
IS  - 1867-0342 (Electronic)
IS  - 1867-0334 (Linking)
VI  - 4
IP  - 3
DP  - 2012 Sep
TI  - Molecular characterization of a complete genome and 12 Nsp2 genes of PRRSV of 
      southwestern China.
PG  - 102-14
LID - 10.1007/s12560-012-9083-z [doi]
AB  - One genome and 12 Nsp2 genes of porcine reproductive and respiratory syndrome 
      virus (PRRSV), isolated from southwestern China during 2009-2010, were sequenced 
      and analyzed. The whole genome comparison analysis revealed that SCwhn09CD is a 
      North American-type PRRSV with separate 30aa deletion in Nsp2 gene, and its 
      sequence highly identical to highly pathogenic PRRSV (HP-PRRSV) outbreak in 2006 
      in almost half area of China. However, an extra 7aa deletion was detected in its 
      Nsp2 coding region. Based on the phylogenetic analysis of Nsp2 gene, all the 12 
      viruses were grouped into North American-type PRRSV, but two in classical-PRRSV 
      (C-PRRSV) subcluster without typical 30aa deletion and ten in HP-PRRSV subcluster 
      with the signature deletion. In addition, many nucleotide substitutions were 
      found in all 12 Nsp2 genes and extra three novel deletions were identified in 
      three out of ten HP-PRRSV. Combining the above data, it may be concluded that: 
      (1) HP-PRRSV and C-PRRSV co-existed in the local pig farms and the majority of 
      the viruses is HR-PRRSV, and (2) the HR-PRRSV continues to undergo its genomic 
      divergence although it does not outbreak in large-scale in recent years. 
      Therefore, continuous surveillance on PRRSV is necessary for the controlling of 
      the virus in this region.
FAU - Zhou, YingShun
AU  - Zhou Y
AD  - School of Life Science, Sichuan University, Animal Disease Prevention and Food 
      Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative 
      Platform for Resource and Environment Protection of Southwestern, Key Laboratory 
      of Bio-resources and Eco-environment, Ministry of Education, Chengdu, 610064, 
      Sichuan, China.
FAU - Yang, Xin
AU  - Yang X
FAU - Wang, Hong-Ning
AU  - Wang HN
FAU - Zhang, AnYun
AU  - Zhang A
FAU - Zhang, ZhiKun
AU  - Zhang Z
FAU - Kang, RunMing
AU  - Kang R
FAU - Zeng, FanYa
AU  - Zeng F
FAU - Li, HaoCheng
AU  - Li H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120712
PL  - United States
TA  - Food Environ Virol
JT  - Food and environmental virology
JID - 101483831
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - Cloning, Molecular
MH  - Gene Deletion
MH  - Genetic Variation
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & 
      purification
MH  - RNA, Viral/genetics/*isolation & purification
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sus scrofa/virology
EDAT- 2013/02/16 06:00
MHDA- 2013/08/30 06:00
CRDT- 2013/02/16 06:00
PHST- 2012/03/10 00:00 [received]
PHST- 2012/06/19 00:00 [accepted]
PHST- 2013/02/16 06:00 [entrez]
PHST- 2013/02/16 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
AID - 10.1007/s12560-012-9083-z [doi]
PST - ppublish
SO  - Food Environ Virol. 2012 Sep;4(3):102-14. doi: 10.1007/s12560-012-9083-z. Epub 
      2012 Jul 12.