PMID- 23415676
OWN - NLM
STAT- MEDLINE
DCOM- 20130826
LR  - 20130318
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1504
DP  - 2013 Apr 4
TI  - Effects of moderate and deep hypothermia on RNA-binding proteins RBM3 and CIRP 
      expressions in murine hippocampal brain slices.
PG  - 74-84
LID - S0006-8993(13)00137-6 [pii]
LID - 10.1016/j.brainres.2013.01.041 [doi]
AB  - Therapeutic hypothermia has emerged as an effective neuroprotective therapy for 
      cardiac arrest survivors. There are a number of purported mechanisms for 
      therapeutic hypothermia, but the exact mechanism still remains to be elucidated. 
      Although hypothermia generally down-regulates protein synthesis and metabolism in 
      mammalian cells, a small subset of homologous (>70%) cold-shock proteins 
      (RNA-binding motif protein 3, RBM3 and cold-inducible RNA-binding protein, CIRP) 
      are induced under these conditions. In addition, RBM3 up-regulation in neuronal 
      cells has recently been implicated in hypothermia-induced neuroprotection. 
      Therefore, we compared the effects of moderate (33.5 degrees C) and deep (17 degrees C) 
      hypothermia with normothermia (37 degrees C) on the regulation of RBM3 and CIRP 
      expressions in murine organotypic hippocampal slice cultures (OHSC), hippocampal 
      neuronal cells (HT-22), and microglia cells (BV-2). Moderate hypothermia resulted 
      in significant up-regulation of both RBM3 and CIRP mRNA in murine OHSC, but deep 
      hyporthermia did not. RBM3 protein regulation was also significantly up-regulated 
      by 33.5 degrees C, but no significant up-regulation of CIRP protein was observed in the 
      OHSC. Additionally, OHSC exposed to 17 degrees C for 24h were positive for Propidium 
      Iodide (PI) immunostaining, indicating the onset of cell death. Similarly, RBM3 
      gene expression in a HT-22 neuronal cells mono-culture and direct co-culture of 
      HT-22 neuronal cells with BV-2 microglia cells were also up-regulated at 33.5 degrees C 
      but only in the co-culture at 17 degrees C. No significant up-regulation of RBM3 nor CIRP 
      gene expression were observed in a BV-2 mono-culture at either temperature. We 
      observed that RBM3 mRNA and protein expressions in murine OHSC, as well as in 
      mono-culture of HT-22 neuronal cells and direct co-culture of HT-22 neuronal 
      cells with BV-2 microglia cells were significantly up-regulated by exposure to 
      moderate hypothermia. These findings further support the implication of RBM3 as a 
      potential effector for hypothermia-induced neuroprotection.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Tong, Giang
AU  - Tong G
AD  - Department of Congenital Heart Disease/Pediatric Cardiology, German Heart 
      Institute Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. 
      giang.tong@charite.de
FAU - Endersfelder, Stefanie
AU  - Endersfelder S
FAU - Rosenthal, Lisa-Maria
AU  - Rosenthal LM
FAU - Wollersheim, Sonja
AU  - Wollersheim S
FAU - Sauer, Igor Maximilian
AU  - Sauer IM
FAU - Buhrer, Christoph
AU  - Buhrer C
FAU - Berger, Felix
AU  - Berger F
FAU - Schmitt, Katharina Rose Luise
AU  - Schmitt KR
LA  - eng
PT  - Journal Article
DEP - 20130208
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Cirbp protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Rbm3 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Coculture Techniques
MH  - Hippocampus/*metabolism
MH  - *Hypothermia, Induced
MH  - Immunoblotting
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/metabolism
MH  - Neurons/metabolism
MH  - Organ Culture Techniques
MH  - RNA-Binding Proteins/*biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Up-Regulation
EDAT- 2013/02/19 06:00
MHDA- 2013/08/27 06:00
CRDT- 2013/02/19 06:00
PHST- 2012/08/07 00:00 [received]
PHST- 2013/01/02 00:00 [revised]
PHST- 2013/01/22 00:00 [accepted]
PHST- 2013/02/19 06:00 [entrez]
PHST- 2013/02/19 06:00 [pubmed]
PHST- 2013/08/27 06:00 [medline]
AID - S0006-8993(13)00137-6 [pii]
AID - 10.1016/j.brainres.2013.01.041 [doi]
PST - ppublish
SO  - Brain Res. 2013 Apr 4;1504:74-84. doi: 10.1016/j.brainres.2013.01.041. Epub 2013 
      Feb 8.