PMID- 23415714
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20141120
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 369
IP  - 1-2
DP  - 2013 Apr 30
TI  - Androgen/androgen receptor pathway regulates expression of the genes for 
      cyclooxygenase-2 and amphiregulin in periovulatory granulosa cells.
PG  - 42-51
LID - S0303-7207(13)00062-2 [pii]
LID - 10.1016/j.mce.2013.02.004 [doi]
AB  - It is well known that the androgen/androgen receptor (AR) pathway is involved in 
      both male and female fertility in mammals. AR knockout female mice are reported 
      to exhibit various abnormalities in follicle development, and a subfertile 
      phenotype. In exogenous gonadotropin-induced superovulation, serum androgen 
      levels were robustly elevated in female mice at the periovulatory stage after 
      human chorionic gonadotropin (hCG) treatment. At this stage, ovarian AR proteins 
      were strongly expressed in cumulus cells. Because these results suggested that 
      the androgen/AR pathway is involved in ovulation, we investigated the expression 
      of ovulation-related genes in the mouse ovary treated with the nonaromatizable 
      androgen, 5alpha-dihydrotestosterone (DHT). DHT treatment induced the expression of 
      the genes for cyclooxyganase-2 (Cox-2 or prostaglandin endoperoxidase synthase 2) 
      and the epidermal growth factor-like factor, amphiregulin (Areg), in the ovary, 
      whereas their hCG-induced expression was suppressed by the AR antagonist 
      flutamide. These genes were also induced by DHT in AR-expressing primary 
      granulosa and granulosa tumor-derived cells. Reporter assays, electrophoretic 
      shift mobility assays and chromatin immunoprecipitation assays demonstrated that 
      androgen response sequence(s) existing upstream of each gene were responsible for 
      androgen responsiveness and were occupied by the AR in periovulatory granulosa 
      cells. Our results suggest that the androgen/AR pathway is involved in the 
      ovulatory process via expression of the Cox-2 and Areg genes in periovulatory 
      granulosa cells.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Yazawa, Takashi
AU  - Yazawa T
AD  - Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, 
      Matsuoka, Fukui, Japan. yazawa@u-fukui.ac.jp
FAU - Kawabe, Shinya
AU  - Kawabe S
FAU - Kanno, Masafumi
AU  - Kanno M
FAU - Mizutani, Tetsuya
AU  - Mizutani T
FAU - Imamichi, Yoshitaka
AU  - Imamichi Y
FAU - Ju, Yunfeng
AU  - Ju Y
FAU - Matsumura, Takehiro
AU  - Matsumura T
FAU - Yamazaki, Yukiko
AU  - Yamazaki Y
FAU - Usami, Yoko
AU  - Usami Y
FAU - Kuribayashi, Mayu
AU  - Kuribayashi M
FAU - Shimada, Masayuki
AU  - Shimada M
FAU - Kitano, Takeshi
AU  - Kitano T
FAU - Umezawa, Akihiro
AU  - Umezawa A
FAU - Miyamoto, Kaoru
AU  - Miyamoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130213
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Androgen Receptor Antagonists)
RN  - 0 (Androgens)
RN  - 0 (Areg protein, mouse)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 76W6J0943E (Flutamide)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Amphiregulin
MH  - Androgen Receptor Antagonists/pharmacology
MH  - Androgens/*pharmacology
MH  - Animals
MH  - Chorionic Gonadotropin/pharmacology
MH  - Cyclooxygenase 2/genetics/*metabolism
MH  - Dihydrotestosterone/*pharmacology
MH  - EGF Family of Proteins
MH  - Female
MH  - Flutamide/pharmacology
MH  - Glycoproteins/genetics/*metabolism
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
EDAT- 2013/02/19 06:00
MHDA- 2013/09/17 06:00
CRDT- 2013/02/19 06:00
PHST- 2012/09/04 00:00 [received]
PHST- 2012/12/26 00:00 [revised]
PHST- 2013/02/05 00:00 [accepted]
PHST- 2013/02/19 06:00 [entrez]
PHST- 2013/02/19 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
AID - S0303-7207(13)00062-2 [pii]
AID - 10.1016/j.mce.2013.02.004 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2013 Apr 30;369(1-2):42-51. doi: 10.1016/j.mce.2013.02.004. 
      Epub 2013 Feb 13.