PMID- 23416263
OWN - NLM
STAT- MEDLINE
DCOM- 20130918
LR  - 20220318
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 27
IP  - 3
DP  - 2013 Apr
TI  - Cerium oxide nanoparticles induce cytotoxicity in human hepatoma SMMC-7721 cells 
      via oxidative stress and the activation of MAPK signaling pathways.
PG  - 1082-8
LID - S0887-2333(13)00028-3 [pii]
LID - 10.1016/j.tiv.2013.02.005 [doi]
AB  - BACKGROUND: Lanthanide cerium oxide (CeO2) nanoparticles have extensive 
      applications in industrial fields, and concerns regarding their potential 
      toxicity in humans and their environmental impact have increased. We investigated 
      the underlying molecular mechanisms by which CeO2 nanoparticles induce toxicity 
      in human hepatoma SMMC-7721 cells. RESULTS: Our results demonstrated that CeO2 
      nanoparticles reduced viability, caused dramatic morphological damage, and 
      induced apoptosis in SMMC-7721 cells. CeO2 nanoparticles significantly increased 
      the production of reactive oxygen species (ROS) and malondialdehyde (MDA), and 
      significantly reduced the activity of superoxide dismutase (SOD), glutathione 
      peroxidase (GSH-px) and catalase (CAT). The phosphorylation levels of ERK1/2, JNK 
      and p38 MAPK were significantly elevated after treatment with CeO2 nanoparticles. 
      Pretreatment with the antioxidant N-acetyl-cysteine (NAC): reduced the induction 
      of ROS and MDA by CeO2 nanoparticles; recovered the activity of SOD, GSH-px and 
      CAT; reduced the phosphorylation levels of ERK1/2, JNK and p38; and attenuated 
      CeO2 nanoparticles-induced damage and apoptosis in SMMC-7721 cells. CONCLUSIONS: 
      Our data demonstrated that CeO2 nanoparticles induced damage and apoptosis in 
      human SMMC-7721 cells via oxidative stress and the activation of MAPK signaling 
      pathways.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Cheng, Guilin
AU  - Cheng G
AD  - Institute of Chemicals Safety, Chinese Academy of Inspection and Quarantine, PR 
      China.
FAU - Guo, Wei
AU  - Guo W
FAU - Han, Lu
AU  - Han L
FAU - Chen, Erlei
AU  - Chen E
FAU - Kong, Lingfang
AU  - Kong L
FAU - Wang, Lili
AU  - Wang L
FAU - Ai, Wenchao
AU  - Ai W
FAU - Song, Naining
AU  - Song N
FAU - Li, Haishan
AU  - Li H
FAU - Chen, Huiming
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130214
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 30K4522N6T (Cerium)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 619G5K328Y (ceric oxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Carcinoma, Hepatocellular
MH  - Catalase/metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cerium/*toxicity
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects
MH  - Malondialdehyde/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Nanoparticles/*toxicity
MH  - Oxidative Stress/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
EDAT- 2013/02/19 06:00
MHDA- 2013/09/21 06:00
CRDT- 2013/02/19 06:00
PHST- 2012/09/10 00:00 [received]
PHST- 2013/01/05 00:00 [revised]
PHST- 2013/02/05 00:00 [accepted]
PHST- 2013/02/19 06:00 [entrez]
PHST- 2013/02/19 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
AID - S0887-2333(13)00028-3 [pii]
AID - 10.1016/j.tiv.2013.02.005 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2013 Apr;27(3):1082-8. doi: 10.1016/j.tiv.2013.02.005. Epub 
      2013 Feb 14.