PMID- 23418612
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20211021
IS  - 1936-5233 (Print)
IS  - 1936-5233 (Electronic)
IS  - 1936-5233 (Linking)
VI  - 6
IP  - 1
DP  - 2013 Feb
TI  - The Reliability of Endoscopic Biopsies in Assessing HER2 Status in Gastric and 
      Gastroesophageal Junction Cancer: A Study Comparing Biopsies with Surgical 
      Samples.
PG  - 10-6
AB  - AIM: The aim of this study is to validate the accuracy of HER2 assessment on 
      biopsies by comparing matched biopsy/surgical material from the same patients. 
      METHODS: HER2 status was evaluated by immunohistochemistry (IHC) and fluorescence 
      in situ hybridization (FISH) in 103 cases of gastric and gastroesophageal 
      junction cancers in coupled biopsy and surgical material. RESULT: Complete 
      concordance between IHC and FISH results on biopsy versus surgical samples was 
      noted in 80% and 95% of cases, respectively. At comprehensive comparison, 
      including IHC and FISH data on biopsy and surgical samples, 89% of biopsies were 
      predictive of HER2 status in surgical samples, whereas 11% showed variable 
      inconsistencies. The majority of these (10 of 12 cases) showed IHC score 0/1+ on 
      biopsy but were all IHC positive and amplified at surgery; in particular, three 
      (3 of 35; 8.5%) IHC score 0 and four (4 of 16; 25%) IHC score 1+ cases were FISH 
      amplified on biopsy material also, whereas the remaining three cases were FISH 
      non-amplified on biopsy. The percentage of cases, which were FISH amplified with 
      IHC score 1+ or 2+ on biopsies, were similar (25% and 33%, respectively) and they 
      also shared a similar grade of amplification. These data suggest that both IHC 
      score 1+ and 2+ on biopsy material represent "equivocal cases" that may merit 
      further investigation. CONCLUSIONS: The predictive value of HER2 IHC in biopsies 
      is high. FISH analysis should be considered for IHC score 2+ and 1+ biopsy cases. 
      Approximately 8% of cases will not be accurately predicted by biopsy evaluation.
FAU - Grillo, Federica
AU  - Grillo F
AD  - Department of Surgical and Diagnostic Sciences (DISC), Pathology Unit, University 
      of Genoa and IRCCS San Martino-IST University Hospital, Genoa, Italy.
FAU - Fassan, Matteo
AU  - Fassan M
FAU - Ceccaroli, Chiara
AU  - Ceccaroli C
FAU - Giacometti, Cinzia
AU  - Giacometti C
FAU - Curto, Monica
AU  - Curto M
FAU - Zagonel, Vittorina
AU  - Zagonel V
FAU - Ceppa, Paola
AU  - Ceppa P
FAU - Nitti, Donato
AU  - Nitti D
FAU - Castoro, Carlo
AU  - Castoro C
FAU - Fiocca, Roberto
AU  - Fiocca R
FAU - Rugge, Massimo
AU  - Rugge M
FAU - Mastracci, Luca
AU  - Mastracci L
LA  - eng
PT  - Journal Article
DEP - 20130201
PL  - United States
TA  - Transl Oncol
JT  - Translational oncology
JID - 101472619
PMC - PMC3573649
EDAT- 2013/02/19 06:00
MHDA- 2013/02/19 06:01
PMCR- 2013/02/01
CRDT- 2013/02/19 06:00
PHST- 2012/10/08 00:00 [received]
PHST- 2012/11/09 00:00 [revised]
PHST- 2012/11/09 00:00 [accepted]
PHST- 2013/02/19 06:00 [entrez]
PHST- 2013/02/19 06:00 [pubmed]
PHST- 2013/02/19 06:01 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - 12334 [pii]
AID - 10.1593/tlo.12334 [doi]
PST - ppublish
SO  - Transl Oncol. 2013 Feb;6(1):10-6. doi: 10.1593/tlo.12334. Epub 2013 Feb 1.