PMID- 23419720
OWN - NLM
STAT- MEDLINE
DCOM- 20130917
LR  - 20211203
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 45
IP  - 4
DP  - 2013 Apr
TI  - Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma 
      HepG2 cells and the sensitivity to cisplatin in vitro.
PG  - 268-79
LID - 10.1093/abbs/gmt006 [doi]
AB  - Mammalian STE20-like kinase 1 (Mst1) is the mammalian homologue of Drosophila 
      Hippo, a major inhibitor of cell proliferation in Drosophila. It ubiquitously 
      encodes serine threonine kinase that belongs to the family of protein kinases 
      related to yeast STE20, and is involved in cell proliferation, apoptosis, 
      oncogenesis, and organ growth. Recent studies have shown that Mst1 has 
      tumor-suppressor function, and the deletion or mutation of Mst1 is reported to be 
      associated with tumorigenesis. To investigate the effect of overexpression of 
      Mst1 on the growth of human liver cancer cell line HepG2 cells and the 
      sensitivity to cisplatin in vitro, here we constructed recombinant eukaryotic 
      expression vector pEGFP-N1-Mst1 containing Mst1 gene, and transiently transfected 
      into HepG2 cells. The effects of Mst1 overexpression on the cell proliferation 
      and apoptosis, the phosphorylation status of Yes-associated protein, and the mRNA 
      transcript levels of connective tissue growth factor (CTGF), amphiregulin (AREG), 
      and birc5 (Survivin) were determined. Results showed that overexpression of Mst1 
      inhibited cell proliferation, induced apoptosis of HepG2 cells, promoted YAP 
      (Ser127) phosphorylation, and downregulated the mRNA expression of CTGF, AREG, 
      and Survivin. We also investigated the relationship between the expression and 
      cleavage of Mst1 and cisplatin-induced cell death. We found that Mst1 
      overexpression could induce cisplatin chemosensitivity, and cisplatin could 
      promote the cleavage of Mst1 without affecting the expression of Mst1. Overall, 
      our results indicated that Mst1 might be a promising anticancer target.
FAU - Xu, Chuanming
AU  - Xu C
AD  - Department of Biochemistry and Molecular Biology, Basic Medical College of 
      Nanchang University, Nanchang 330006, China.
FAU - Liu, Chunju
AU  - Liu C
FAU - Huang, Wei
AU  - Huang W
FAU - Tu, Shuo
AU  - Tu S
FAU - Wan, Fusheng
AU  - Wan F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130217
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (AREG protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Amphiregulin)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (BIRC5 protein, human)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Survivin)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 2.7.1.11 (STK4 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
EIN - Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):801
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Amphiregulin
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Carcinoma, Hepatocellular/genetics/metabolism/pathology
MH  - Cell Proliferation/*drug effects
MH  - Cisplatin/*pharmacology
MH  - Connective Tissue Growth Factor/genetics/metabolism
MH  - Down-Regulation/drug effects
MH  - EGF Family of Proteins
MH  - Gene Expression Regulation, Neoplastic
MH  - Glycoproteins/genetics/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Liver Neoplasms/genetics/metabolism/pathology
MH  - Microscopy, Fluorescence
MH  - Phosphoproteins/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Survivin
MH  - Transcription Factors
MH  - YAP-Signaling Proteins
EDAT- 2013/02/20 06:00
MHDA- 2013/09/18 06:00
CRDT- 2013/02/20 06:00
PHST- 2013/02/20 06:00 [entrez]
PHST- 2013/02/20 06:00 [pubmed]
PHST- 2013/09/18 06:00 [medline]
AID - gmt006 [pii]
AID - 10.1093/abbs/gmt006 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2013 Apr;45(4):268-79. doi: 
      10.1093/abbs/gmt006. Epub 2013 Feb 17.