PMID- 23420617
OWN - NLM
STAT- MEDLINE
DCOM- 20140128
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 36
IP  - 5
DP  - 2013
TI  - 6,4'-Dihydroxy-7-methoxyflavanone inhibits osteoclast differentiation and 
      function.
PG  - 796-801
AB  - 6,4'-Dihydroxy-7-methoxyflavanone (DMF) is a flavonoid isolated from Heartwood 
      Dalbergia odorifera. It has been known that DMF has antioxidant, 
      anti-inflammatory and neuroprotective effects. DMF, however, the efficacy of bone 
      related diseases has not been reported. In this study, we determined DMF's 
      efficacy on osteoclasts differentiation and function using in vitro bone marrow 
      macrophage osteoclast differentiation culture system. DMF inhibited receptor 
      activators of nuclear factor kappa-B ligand (RANKL) induced osteoclastogenesis 
      dose dependently. In addition, DMF decreased osteoclast function through 
      disruption of actin ring formation and consequently suppression of the 
      pit-forming activity of mature osteoclasts. Mechanistically, DMF inhibited 
      RANKL-induced expression of nuclear factor of activated T-cells, cytoplasmic, 
      calcineurin-dependent 1 (NFATc1) and c-Fos via inhibition of mitogen activated 
      protein kinases (MAPKs) pathway. Collectively, the inhibition of osteoclasts 
      differentiation and function by DMF suggests that DMF can be a potential 
      therapeutic molecule for osteoclastogenic bone diseases such osteoporosis, 
      rheumatoid arthritis and periodontal diseases.
FAU - Im, Nam-Kyung
AU  - Im NK
AD  - Institute for New Drug Development, Keimyung University, Dae-gu 704-701, Republic 
      of Korea.
FAU - Choi, Je-Yong
AU  - Choi JY
FAU - Oh, Hyuncheol
AU  - Oh H
FAU - Kim, Youn-Chul
AU  - Kim YC
FAU - Jeong, Gil-Saeng
AU  - Jeong GS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130219
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (6,4'-dihydroxy-7-methoxyflavanone)
RN  - 0 (Actins)
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Flavanones)
RN  - 0 (RANK Ligand)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bone Density Conservation Agents/*pharmacology
MH  - Bone Marrow Cells/cytology
MH  - Bone Resorption/*drug therapy/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Flavanones/*pharmacology
MH  - Macrophages/cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Osteoclasts/cytology/*drug effects/physiology
MH  - RANK Ligand/pharmacology
EDAT- 2013/02/20 06:00
MHDA- 2014/01/29 06:00
CRDT- 2013/02/20 06:00
PHST- 2013/02/20 06:00 [entrez]
PHST- 2013/02/20 06:00 [pubmed]
PHST- 2014/01/29 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b12-00964 [pii]
AID - 10.1248/bpb.b12-00964 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2013;36(5):796-801. doi: 10.1248/bpb.b12-00964. Epub 2013 Feb 
      19.