PMID- 23420667
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 5
IP  - 2
DP  - 2013 Feb
TI  - The mTOR inhibitor AZD8055 inhibits proliferation and glycolysis in cervical 
      cancer cells.
PG  - 717-721
AB  - The aim of the present study was to determine the effect of AZD8055 on 
      proliferation, apoptosis and glycolysis in the human cervical cancer cell line 
      HeLa and to investigate the underlying mechanism(s) of action. HeLa human 
      cervical cancer cells were treated with 10 nM AZD8055 for 24, 48 or 72 h. MTT was 
      used to determine cell proliferation. Annexin V/propidium iodide staining was 
      used to determine cell apoptosis analyzed by fluorescence-activated cell sorting 
      (FACS). Glycolytic activity was determined by measuring the activity of the key 
      enzyme lactate dehydrogenase (LDH) and lactate production. RNA and protein 
      expression were examined by qRT-PCR and western blotting, respectively. Treatment 
      with AZD8055 inhibited proliferation and glycolysis, and induced apoptosis in 
      HeLa cells in a time-dependent manner. During the prolonged treatment with 
      AZD8055, the phosphorylation of mammalian target of rapamycin (mTOR) C1 
      substrates p70S6K and phosphorylation of the mTORC2 substrate Akt were 
      deregulated, suggesting that the activity of mTOR was downregulated. Furthermore, 
      our study showed that the expression of miR-143 was upregulated in a 
      time-dependent manner in HeLa cells treated with AZD8055. In summary, the present 
      study reveals a novel antitumor mechanism of AZD8055 in HeLa human cervical 
      cancer cells.
FAU - Li, Shaoru
AU  - Li S
AD  - Departments of Gynecology and Obstetrics, The First Affiliated Hospital of 
      Xinxiang Medical University, Weihui, Henan 453100;
FAU - Li, Yan
AU  - Li Y
FAU - Hu, Ruili
AU  - Hu R
FAU - Li, Weihua
AU  - Li W
FAU - Qiu, Haifeng
AU  - Qiu H
FAU - Cai, Honghua
AU  - Cai H
FAU - Wang, Shijin
AU  - Wang S
LA  - eng
PT  - Journal Article
DEP - 20121204
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3573114
OTO - NOTNLM
OT  - AZD8055
OT  - anticancer activity
OT  - cervical cancer
OT  - glycolysis
OT  - mTOR signaling
OT  - proliferation
EDAT- 2013/02/20 06:00
MHDA- 2013/02/20 06:01
PMCR- 2012/12/04
CRDT- 2013/02/20 06:00
PHST- 2012/10/11 00:00 [received]
PHST- 2012/11/20 00:00 [accepted]
PHST- 2013/02/20 06:00 [entrez]
PHST- 2013/02/20 06:00 [pubmed]
PHST- 2013/02/20 06:01 [medline]
PHST- 2012/12/04 00:00 [pmc-release]
AID - ol-05-02-0717 [pii]
AID - 10.3892/ol.2012.1058 [doi]
PST - ppublish
SO  - Oncol Lett. 2013 Feb;5(2):717-721. doi: 10.3892/ol.2012.1058. Epub 2012 Dec 4.