PMID- 2342248
OWN - NLM
STAT- MEDLINE
DCOM- 19900625
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 37
IP  - 4
DP  - 1990 Apr
TI  - C5b-8 and C5b-9 modulate the collagen release of human glomerular epithelial 
      cells.
PG  - 1098-104
AB  - Aside from their lytic function the late complement components C5b-9 stimulate 
      release of prostanoids, interleukin 1 and oxygen radicals from a number of cells. 
      Since C5b-9 has also been connected to the development of sclerosis in animal 
      models of glomerulonephritis, we addressed the question whether C5b-9 would 
      affect the collagen synthesis. We used human glomerular epithelial cells (GEC) 
      obtained as primary outgrowth cultures. The cells were cultivated in the presence 
      of 14C-proline. Collagen synthesis was quantitated by counting the radioactivity 
      associated with collagenase digestible material. Furthermore, collagen was 
      analyzed by SDS-PAGE. GEC in culture produce spontaneously some collagen type IV. 
      Addition of sublytic doses of highly purified C5b-9 increased the collagen 
      synthesis considerably within 12 to 24 hours. In the absence of C9, C5b-8 
      stimulated collagen synthesis to a similar extent, whereas in the absence of C7 
      or C8, the collagen synthesis was not enhanced. Furthermore, fluid-phase-formed 
      C5b-9 complexes did not stimulate the collagen synthesis, indicating that 
      assembly of the complex on the target membrane was required. Since C5b-9 deposits 
      are found in sclerotic areas, our data support the hypothesis that C5b-9, by 
      stimulating collagen synthesis as well as release, might contribute to the 
      development of chronic nephritis.
FAU - Torbohm, I
AU  - Torbohm I
AD  - Institut fur Immunologie, Universitat Heidelberg, Federal Republic of Germany.
FAU - Schonermark, M
AU  - Schonermark M
FAU - Wingen, A M
AU  - Wingen AM
FAU - Berger, B
AU  - Berger B
FAU - Rother, K
AU  - Rother K
FAU - Hansch, G M
AU  - Hansch GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Complement Membrane Attack Complex)
RN  - 0 (complement C5b-8 complex)
RN  - 9007-34-5 (Collagen)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen/biosynthesis/*metabolism
MH  - Complement Membrane Attack Complex/metabolism/*pharmacology
MH  - Complement System Proteins/metabolism/*pharmacology
MH  - Epithelium/drug effects/metabolism
MH  - Humans
MH  - Kidney Glomerulus/drug effects/*metabolism
MH  - Rats
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
AID - S0085-2538(15)34856-0 [pii]
AID - 10.1038/ki.1990.91 [doi]
PST - ppublish
SO  - Kidney Int. 1990 Apr;37(4):1098-104. doi: 10.1038/ki.1990.91.