PMID- 23424650
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20220317
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 2
DP  - 2013
TI  - Increased levels of IgG antibodies against human HSP60 in patients with 
      spondyloarthritis.
PG  - e56210
LID - 10.1371/journal.pone.0056210 [doi]
LID - e56210
AB  - Spondyloarthritis (SpA) comprises a heterogeneous group of inflammatory diseases, 
      with strong association to human leukocyte antigen (HLA)-B27. A triggering 
      bacterial infection has been considered as the cause of SpA, and bacterial heat 
      shock protein (HSP) seems to be a strong T cell antigen. Since bacterial and 
      human HSP60, also named HSPD1, are highly homologous, cross-reactivity has been 
      suggested in disease initiation. In this study, levels of antibodies against 
      bacterial and human HSP60 were analysed in SpA patients and healthy controls, and 
      the association between such antibodies and disease severity in relation to 
      HLA-B27 was evaluated.Serum samples from 82 patients and 50 controls were 
      analysed by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (Ig)G1, 
      IgG2, IgG3 and IgG4 antibodies against human HSP60 and HSP60 from Chlamydia 
      trachomatis, Salmonella enteritidis and Campylobacter jejuni. Disease severity 
      was assessed by the clinical scorings Bath Ankylosing Spondylitis Disease 
      Activity Index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI) and 
      Bath Ankylosing Spondylitis Metrology Index (BASMI). Levels of IgG1 and IgG3 
      antibodies against human HSP60, but not antibodies against bacterial HSP60, were 
      elevated in the SpA group compared with the control group. Association between 
      IgG3 antibodies against human HSP60 and BASMI was shown in HLA-B27(+) patients. 
      Only weak correlation between antibodies against bacterial and human HSP60 was 
      seen, and there was no indication of cross-reaction. These results suggest that 
      antibodies against human HSP60 is associated with SpA, however, the theory that 
      antibodies against human HSP60 is a specific part of the aetiology, through 
      cross-reaction to bacterial HSP60, cannot be supported by results from this 
      study. We suggest that the association between elevated levels of antibodies 
      against human HSP60 and disease may reflect a general activation of the immune 
      system and an increased expression of human HSP60 in the synovium of patients 
      with SpA.
FAU - Hjelholt, Astrid
AU  - Hjelholt A
AD  - Department of Biomedicine-Medical Microbiology and Immunology, Aarhus University, 
      Aarhus, Denmark. astrid.hjelholt@studmed.au.dk
FAU - Carlsen, Thomas
AU  - Carlsen T
FAU - Deleuran, Bent
AU  - Deleuran B
FAU - Jurik, Anne Grethe
AU  - Jurik AG
FAU - Schiottz-Christensen, Berit
AU  - Schiottz-Christensen B
FAU - Christiansen, Gunna
AU  - Christiansen G
FAU - Birkelund, Svend
AU  - Birkelund S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130212
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Chaperonin 60)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Bacterial/blood
MH  - Case-Control Studies
MH  - Chaperonin 60/*immunology
MH  - Female
MH  - HLA-B27 Antigen/immunology
MH  - Humans
MH  - Immunoglobulin G/*blood/*immunology
MH  - Male
MH  - Middle Aged
MH  - Spondylarthritis/*blood/*immunology
MH  - Young Adult
PMC - PMC3570413
COIS- Competing Interests: SB and GC are shareholders in Loke Diagnostics, Risskov, 
      Denmark, which provided the HSP60 used in this study. This does not alter the 
      authors' adherence to all the PLOS ONE policies on sharing data and materials.
EDAT- 2013/02/21 06:00
MHDA- 2013/08/09 06:00
PMCR- 2013/02/12
CRDT- 2013/02/21 06:00
PHST- 2012/05/25 00:00 [received]
PHST- 2013/01/10 00:00 [accepted]
PHST- 2013/02/21 06:00 [entrez]
PHST- 2013/02/21 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
PHST- 2013/02/12 00:00 [pmc-release]
AID - PONE-D-12-15852 [pii]
AID - 10.1371/journal.pone.0056210 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(2):e56210. doi: 10.1371/journal.pone.0056210. Epub 2013 Feb 12.