PMID- 23424873
OWN - NLM
STAT- MEDLINE
DCOM- 20131219
LR  - 20130221
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 41
IP  - 6
DP  - 2012 Nov
TI  - [Anti-tumor effect of phytic acid on human osteosarcoma U2OS cells in vitro].
PG  - 943-6
AB  - OBJECTIVE: To explore the effect of phytic acid on the human osteosarcoma U20S 
      cells and its mechanisms in vitro. METHODS: MTT assay was used to examine the 
      cell proliferation. Scanning electron microscope (SEM) was used to observe the 
      surface ultrastructure of U20S cells. Hoechst 33342 fluorescence staining assay 
      were applied to study the pro-apoptosis effects. The immune histochemisty method 
      was used to detect c-myc expression. RESULTS: Phytic acid could significantly 
      inhibit the growth of U20S. The surface ultrastructure of U20S cells showed 
      distinct morphological changes corresponding to a typical cellular surface 
      morphology of apoptosis. From fluorescence staining, we observed chromoplasm 
      pyknosis and apoptotic body. Expression of c-myc protein decreased. CONCLUSION: 
      The anti-osteosarcoma mechanism of phytic acid may be related to its influence on 
      inducing apoptosis and adjusting c-myc expression.
FAU - Wang, Lu
AU  - Wang L
AD  - School of Food Science and Engineering, Harbin Institute of Technology, Harbin 
      150090, China. wanglu_tianfeng@yahoo.com.cn
FAU - Cheng, Cuilin
AU  - Cheng C
FAU - Zhao, Haitian
AU  - Zhao H
FAU - Cui, Hongbin
AU  - Cui H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Antineoplastic Agents)
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 7IGF0S7R8I (Phytic Acid)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Bone Neoplasms/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - Osteosarcoma/*pathology
MH  - Phytic Acid/*pharmacology
MH  - Proto-Oncogene Proteins c-myc/drug effects
EDAT- 2013/02/22 06:00
MHDA- 2013/12/20 06:00
CRDT- 2013/02/22 06:00
PHST- 2013/02/22 06:00 [entrez]
PHST- 2013/02/22 06:00 [pubmed]
PHST- 2013/12/20 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2012 Nov;41(6):943-6.