PMID- 23424876
OWN - NLM
STAT- MEDLINE
DCOM- 20131219
LR  - 20130221
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 41
IP  - 6
DP  - 2012 Nov
TI  - [Simultaneous determination of nine estrogens in eel by ultrafast liquid 
      chromatography-tandem mass spectrometry with isotope dilution technique and 
      solid-phase extraction].
PG  - 956-62
AB  - OBJECTIVE: Developing a rapid and sensitive analytical method based on ultrafast 
      liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) with solid-phase 
      extraction (SPE) for the simultaneous determination of nine estrogens 
      (dienestrol, diethylstilbestrol, estrone, hexestrol, 17-alpha-estradiol, 
      17-beta-estradiol, estriol, 17alpha-ethinylestradiol and estradiol valerate) in 
      eel. METHODS: After the sample was extracted by acetonitrile and cleaned by 
      Waters Oasis HLB solid-phase extraction cartridge, the UFLC separation was 
      performed on a Shim-pack XR-ODS II column (100 mm x 2.0 mm, 2.2 microm) with a 
      linear gradient elution program of methanol solution containing 0.04% ammonia 
      (v/v) and 0.04% ammonia aqueous solution (v/v) as the mobile phase. Electrospray 
      ionization was applied and operated in the negative multiple reaction monitoring 
      (MRM) mode. The quantitation was used by isotope internal standard dilution 
      technique. RESULTS: The results showed that the limits of quantitation (LOQs, 
      S/N(10) were in the range of 0.07-0.4 microg/kg, the calibration curves were in 
      good linearities for the nine analytes in the range of 0.5-50.0 microg/L with the 
      correlative coefficients (r2) more than 0.998, the recoveries were between 81.0% 
      and 110.0% with the relative standard deviations (RSDs) of 1.92%-8.24%. 
      Additional, the mass spectra characterization of the nine estrogens was discussed 
      and the fragmentation pathways were speculated. CONCLUSION: The developed method 
      is rapid, sensitive, specific and reproducible, and adapts not only to the 
      simultaneous determination of the nine trace estrogens including the epimer of 
      17-alpha-estradiol and 17-beta-estradiol but also to the identified detection in 
      other fish tissues.
FAU - Chen, Xiaohong
AU  - Chen X
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory 
      of Poison Research and Control, Ningbo 315010, China. chenxhnb@163.com
FAU - Yao, Shanshan
AU  - Yao S
FAU - Li, Xiaoping
AU  - Li X
FAU - Zhao, Yonggang
AU  - Zhao Y
FAU - Jin, Micong
AU  - Jin M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Estrogens)
RN  - 2DI9HA706A (Estrone)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - RRW32X4U1F (Dienestrol)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Dienestrol/analysis
MH  - Diethylstilbestrol/analysis
MH  - *Eels
MH  - Estrogens/*analysis
MH  - Estrone/analysis
MH  - Fish Products/*analysis
MH  - Food Contamination/analysis
MH  - Radioisotope Dilution Technique
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/02/22 06:00
MHDA- 2013/12/20 06:00
CRDT- 2013/02/22 06:00
PHST- 2013/02/22 06:00 [entrez]
PHST- 2013/02/22 06:00 [pubmed]
PHST- 2013/12/20 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2012 Nov;41(6):956-62.