PMID- 23425507
OWN - NLM
STAT- MEDLINE
DCOM- 20140210
LR  - 20161020
IS  - 1469-5111 (Electronic)
IS  - 1461-1457 (Linking)
VI  - 16
IP  - 7
DP  - 2013 Aug
TI  - BDNF parabrachio-amygdaloid pathway in morphine-induced analgesia.
PG  - 1649-60
LID - 10.1017/S146114571200168X [doi]
AB  - In addition to its neurotrophic role, brain-derived neurotrophic factor (BDNF) is 
      involved in a wide array of functions, including anxiety and pain. The central 
      amygdaloid nucleus (CeA) contains a high concentration of BDNF in terminals, 
      originating from the pontine parabrachial nucleus. Since the 
      spino-parabrachio-amygdaloid neural pathway is known to convey nociceptive 
      information, we hypothesized a possible involvement of BDNF in supraspinal 
      pain-related processes. To test this hypothesis, we generated localized deletion 
      of BDNF in the parabrachial nucleus using local bilateral injections of 
      adeno-associated viruses in adult floxed-BDNF mice. Basal thresholds of thermal 
      and mechanical nociceptive responses were not altered by BDNF loss and no 
      behavioural deficit was noticed in anxiety and motor tests. However, BDNF-deleted 
      animals displayed a major decrease in the analgesic effect of morphine. In 
      addition, intra-CeA injections of the BDNF scavenger TrkB-Fc in control mice also 
      decreased morphine-induced analgesia. Finally, the number of c-Fos immunoreactive 
      nuclei after acute morphine injection was decreased by 45% in the extended 
      amygdala of BDNF-deleted animals. The absence of BDNF in the parabrachial nucleus 
      thus altered the parabrachio-amygdaloid pathway. Overall, our study provides 
      evidence that BDNF produced in the parabrachial nucleus modulates the functions 
      of the parabrachio-amygdaloid pathway in opiate analgesia.
FAU - Sarhan, Maysa
AU  - Sarhan M
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Pawlowski, Sophie Anne
AU  - Pawlowski SA
FAU - Barthas, Florent
AU  - Barthas F
FAU - Yalcin, Ipek
AU  - Yalcin I
FAU - Kaufling, Jennifer
AU  - Kaufling J
FAU - Dardente, Hugues
AU  - Dardente H
FAU - Zachariou, Venetia
AU  - Zachariou V
FAU - Dileone, Ralph Joseph
AU  - Dileone RJ
FAU - Barrot, Michel
AU  - Barrot M
FAU - Veinante, Pierre
AU  - Veinante P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130221
PL  - England
TA  - Int J Neuropsychopharmacol
JT  - The international journal of neuropsychopharmacology
JID - 9815893
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Amygdala/drug effects/*metabolism
MH  - Analgesics, Opioid/*pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Dark Adaptation/drug effects
MH  - Dependovirus/genetics
MH  - Exploratory Behavior/drug effects
MH  - Hyperalgesia/drug therapy
MH  - Male
MH  - Maze Learning/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Morphine/*pharmacology
MH  - Neural Pathways/drug effects
MH  - Pain Measurement
MH  - Pain Threshold/drug effects
MH  - Pons/drug effects/*metabolism
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Rotarod Performance Test
EDAT- 2013/02/22 06:00
MHDA- 2014/02/11 06:00
CRDT- 2013/02/22 06:00
PHST- 2013/02/22 06:00 [entrez]
PHST- 2013/02/22 06:00 [pubmed]
PHST- 2014/02/11 06:00 [medline]
AID - S146114571200168X [pii]
AID - 10.1017/S146114571200168X [doi]
PST - ppublish
SO  - Int J Neuropsychopharmacol. 2013 Aug;16(7):1649-60. doi: 
      10.1017/S146114571200168X. Epub 2013 Feb 21.