PMID- 23427087
OWN - NLM
STAT- MEDLINE
DCOM- 20150715
LR  - 20150107
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 37
IP  - 4
DP  - 2013 Apr
TI  - Effect of treadmill running on the expression of genes that are involved in 
      neuronal differentiation in the hippocampus of adult male rats.
PG  - 276-83
AB  - Gene expression in the rodent brain is dependent on exercise. However, our 
      findings regarding this fact are inadequate. The aim of this study was to 
      evaluate the effect of exercise intensity on changes in factors involved in 
      neuronal differentiation and plasticity in the hippocampus of adult male rats. 
      Thus, the effects of 2 weeks treadmill running at of low (11 m/min) and high (25 
      m/min) intensities, 30 min/day for 14 consecutive days on the expression of two 
      genes, microRNA-124 and neuron restrictive silencer factor/RE1-silencing 
      transcription factor (REST) as well as changes of two proteins of brain-derived 
      eurotrophic factor (BDNF) and tyrosine kinase B (TrkB) were analysed. Using 
      quantitative real-time PCR techniques have shown that both exercise intensities 
      reduced the expression of REST (31% in low exercise intensity, 52% in high 
      exercise intensity) and elevated the expression of miR-124 (3.8-fold in low 
      exercise intensity, 11.9-fold in high exercise intensity)significantly (P</=0.05). 
      This suggests that exercise probably through the changes in these genes is 
      involved in neuronal differentiation in the hippocampus of rats. BDNF and TrkB 
      levels were measured by ELISA. Exercise, at low intensity, increased TrkB (4.51 
      ng/L vs. 3.73 ng/L) and BDNF (11.97 ng/L vs. 11.08 ng/L) proteins significantly 
      (P</=0.05), while at high intensity,the changes in comparison with the control 
      group were not significant. Expression levels during the exercise programme with 
      high intensity were dramatic for miR-124 and less dramatic for REST compared with 
      low intensity exercise programme.
FAU - Mojtahedi, Shima
AU  - Mojtahedi S
AD  - Department of Exercise Physiology, Faculty of Physical Education and Sport 
      Sciences, University of Tehran, Tehran, Iran
FAU - Kordi, Mohammad Reza
AU  - Kordi MR
FAU - Hosseini, Seyed Ebrahim
AU  - Hosseini SE
FAU - Omran, Simin Fallah
AU  - Omran SF
FAU - Soleimani, Masoud
AU  - Soleimani M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN124 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (Repressor Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - *Cell Differentiation
MH  - Gene Expression
MH  - Hippocampus/*cytology
MH  - Male
MH  - MicroRNAs/metabolism
MH  - Neurons/*physiology
MH  - Physical Conditioning, Animal
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/metabolism
MH  - Repressor Proteins/genetics/metabolism
MH  - Running
EDAT- 2013/02/22 06:00
MHDA- 2015/07/16 06:00
CRDT- 2013/02/22 06:00
PHST- 2012/11/15 00:00 [received]
PHST- 2012/11/15 00:00 [accepted]
PHST- 2013/02/22 06:00 [entrez]
PHST- 2013/02/22 06:00 [pubmed]
PHST- 2015/07/16 06:00 [medline]
AID - 10.1002/cbin.10022 [doi]
PST - ppublish
SO  - Cell Biol Int. 2013 Apr;37(4):276-83. doi: 10.1002/cbin.10022.