PMID- 23429425
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130225
LR  - 20211021
IS  - 2046-6390 (Print)
IS  - 2046-6390 (Electronic)
IS  - 2046-6390 (Linking)
VI  - 2
IP  - 2
DP  - 2013 Feb 15
TI  - Expression of claudins -2 and -4 and cingulin is coordinated with the start of 
      stratification and differentiation in corneal epithelial cells: retinoic acid 
      reversibly disrupts epithelial barrier.
PG  - 132-43
LID - 10.1242/bio.20123145 [doi]
AB  - Although tight junctions (TJ) have been extensively studied in simple epithelial 
      cells, it is still unknown whether their organization is coupled to cell 
      differentiation in stratified epithelia. We studied the expression of TJ in 
      RCE1(5T5) cells, an in vitro model which mimics the sequential steps of rabbit 
      corneal epithelial differentiation. RCE1(5T5) cells expressed TJ components which 
      were assembled once cells constituted differentiated epithelia, as suggested by 
      the increase of transepithelial electrical resistance (TER) which followed a 
      similar kinetic to the expression of the early differentiation marker Pax-6. TJ 
      were functional as indicated by the establishment of an epithelial barrier 
      nonpermeable to ruthenium red or a biotin tracer. In immunostaining experiments, 
      TJ were located at the superficial cells from the suprabasal layers; Western blot 
      and RT-PCR suggested that TJ were composed of claudins (cldn) -1, -2, -4, 
      cingulin (cgn), occludin (ocln) and ZO-1. Semi-quantitative RT-PCR and TER 
      measurements showed that TJ became organized when cells began to form a 3-5 
      layers stratified epithelium; TER increased once cells reached confluence, with a 
      time course comparable to the raise in the expression of cgn, cldn-2 and -4. 
      Nevertheless, cldn-1, -2, ZO-1 and ocln were present in the cells from the 
      beginning of cultivation, suggesting that TER increases mainly depend on TJ 
      assembly. While EGF increased epithelial barrier strength, retinoic acid 
      disrupted it, increasing paracellular flux about 2-fold; this effect was 
      concentration dependent and completely reversible. Our results suggest that TJ 
      assembly is tightly linked to the expression of corneal epithelial terminal 
      phenotype.
FAU - Ortiz-Melo, Maria Teresa
AU  - Ortiz-Melo MT
AD  - Departamento de Biologia Celular, Centro de Investigacion y de Estudios Avanzados 
      del Instituto Politecnico Nacional , Apartado Postal 14-740, Mexico City 07000 , 
      Mexico ; Posgrado en Ciencias Biologicas, FES-Iztacala, Universidad Nacional 
      Autonoma de Mexico , Mexico City 04510 , Mexico.
FAU - Sanchez-Guzman, Erika
AU  - Sanchez-Guzman E
FAU - Gonzalez-Robles, Arturo
AU  - Gonzalez-Robles A
FAU - Valdes, Jesus
AU  - Valdes J
FAU - Gomez-Flores, Eber
AU  - Gomez-Flores E
FAU - Castro-Munozledo, Federico
AU  - Castro-Munozledo F
LA  - eng
PT  - Journal Article
DEP - 20121120
PL  - England
TA  - Biol Open
JT  - Biology open
JID - 101578018
PMC - PMC3575648
OTO - NOTNLM
OT  - Cell culture
OT  - Cell differentiation
OT  - Cingulin
OT  - Claudin
OT  - Corneal epithelium
OT  - Occludin
OT  - Retinoic acid
OT  - Tight junction
COIS- Competing interests: The authors have no competing interests to declare.
EDAT- 2013/02/23 06:00
MHDA- 2013/02/23 06:01
PMCR- 2012/11/20
CRDT- 2013/02/23 06:00
PHST- 2012/09/18 00:00 [received]
PHST- 2012/10/29 00:00 [accepted]
PHST- 2013/02/23 06:00 [entrez]
PHST- 2013/02/23 06:00 [pubmed]
PHST- 2013/02/23 06:01 [medline]
PHST- 2012/11/20 00:00 [pmc-release]
AID - BIO20123145 [pii]
AID - 10.1242/bio.20123145 [doi]
PST - ppublish
SO  - Biol Open. 2013 Feb 15;2(2):132-43. doi: 10.1242/bio.20123145. Epub 2012 Nov 20.