PMID- 23430249
OWN - NLM
STAT- MEDLINE
DCOM- 20130607
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 288
IP  - 15
DP  - 2013 Apr 12
TI  - Natural HLA-B*2705 protein ligands with glutamine as anchor motif: implications 
      for HLA-B27 association with spondyloarthropathy.
PG  - 10882-9
LID - 10.1074/jbc.M113.455352 [doi]
AB  - The presentation of short viral peptide antigens by human leukocyte antigen (HLA) 
      class I molecules on cell surfaces is a key step in the activation of cytotoxic T 
      lymphocytes, which mediate the killing of pathogen-infected cells or initiate 
      autoimmune tissue damage. HLA-B27 is a well known class I molecule that is used 
      to study both facets of the cellular immune response. Using mass spectrometry 
      analysis of complex HLA-bound peptide pools isolated from large amounts of 
      HLA-B*2705(+) cells, we identified 200 naturally processed HLA-B*2705 ligands. 
      Our analyses revealed that a change in the position (P) 2 anchor motif was 
      detected in the 3% of HLA-B*2705 ligands identified. B*2705 class I molecules 
      were able to bind these six GlnP2 peptides, which showed significant homology to 
      pathogenic bacterial sequences, with a broad range of affinities. One of these 
      ligands was able to bind with distinct conformations to HLA-B27 subtypes 
      differentially associated with ankylosing spondylitis. These conformational 
      differences could be sufficient to initiate autoimmune damage in patients with 
      ankylosing spondylitis-associated subtypes. Therefore, these kinds of peptides 
      (short, with GlnP2, and similar low affinity to all HLA-B27 subtypes tested but 
      with unlike conformations in differentially ankylosing spondylitis-associated 
      subtypes) must not be excluded from future researches involving potential 
      arthritogenic peptides.
FAU - Infantes, Susana
AU  - Infantes S
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda (Madrid), Spain.
FAU - Lorente, Elena
AU  - Lorente E
FAU - Barnea, Eilon
AU  - Barnea E
FAU - Beer, Ilan
AU  - Beer I
FAU - Barriga, Alejandro
AU  - Barriga A
FAU - Lasala, Fatima
AU  - Lasala F
FAU - Jimenez, Mercedes
AU  - Jimenez M
FAU - Admon, Arie
AU  - Admon A
FAU - Lopez, Daniel
AU  - Lopez D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130219
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (HLA-B*27:05 antigen)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Motifs
MH  - Cell Line
MH  - HLA-B27 Antigen/genetics/*immunology/metabolism
MH  - Humans
MH  - Peptides/genetics/*immunology/metabolism
MH  - Protein Binding/genetics/immunology
MH  - Spondylarthropathies/genetics/*immunology/metabolism/pathology
PMC - PMC3624468
EDAT- 2013/02/23 06:00
MHDA- 2013/06/08 06:00
PMCR- 2014/04/12
CRDT- 2013/02/23 06:00
PHST- 2013/02/23 06:00 [entrez]
PHST- 2013/02/23 06:00 [pubmed]
PHST- 2013/06/08 06:00 [medline]
PHST- 2014/04/12 00:00 [pmc-release]
AID - S0021-9258(20)67310-0 [pii]
AID - M113.455352 [pii]
AID - 10.1074/jbc.M113.455352 [doi]
PST - ppublish
SO  - J Biol Chem. 2013 Apr 12;288(15):10882-9. doi: 10.1074/jbc.M113.455352. Epub 2013 
      Feb 19.