PMID- 23432085 OWN - NLM STAT- MEDLINE DCOM- 20130701 LR - 20131121 IS - 1471-4159 (Electronic) IS - 0022-3042 (Linking) VI - 125 IP - 4 DP - 2013 May TI - Brain-derived neurotrophic factor, corticotropin-releasing factor, and hypothalamic neuronal histamine interact to regulate feeding behavior. PG - 588-98 LID - 10.1111/jnc.12213 [doi] AB - Brain-derived neurotrophic factor (BDNF), corticotropin-releasing factor (CRF), and hypothalamic neuronal histamine are anorexigenic substances within the hypothalamus. This study examined the interactions among BDNF, CRF, and histamine during the regulation of feeding behavior in rodents. Food intake was measured after treatment with BDNF, alpha-fluoromethyl histidine (FMH; a specific suicide inhibitor of histidine decarboxylase that depletes hypothalamic neuronal histamine), or CRF antagonist. We measured food intake in wild-type mice and mice with targeted disruption of the histamine H1 receptor (H1KO mice) after central BDNF infusion. Furthermore, we investigated CRF content and histamine turnover in the hypothalamus after BDNF treatment, and conversely, BDNF content in the hypothalamus after histamine treatment. We used immunohistochemical staining for histamine H1 receptors (H1-R) in BDNF neurons. BDNF-induced feeding suppression was partially attenuated in rats pre-treated with FMH or a CRF antagonist, and in H1KO mice. BDNF treatment increased CRF content and histamine turnover in the hypothalamus. Histamine increased BDNF content in the hypothalamus. Immunohistochemical analysis revealed that H1-Rs were expressed on BDNF neurons in the ventromedial nucleus of the hypothalamus. These results indicate that CRF and hypothalamic neuronal histamine mediate the suppressive effects of BDNF on feeding behavior and body weight. CI - (c) 2013 International Society for Neurochemistry. FAU - Gotoh, Koro AU - Gotoh K AD - Department of Internal Medicine 1, Faculty of Medicine, Oita University, Yufu, Japan. gotokoro@oita-u.ac.jp FAU - Masaki, Takayuki AU - Masaki T FAU - Chiba, Seiichi AU - Chiba S FAU - Ando, Hisae AU - Ando H FAU - Fujiwara, Kansuke AU - Fujiwara K FAU - Shimasaki, Takanobu AU - Shimasaki T FAU - Mitsutomi, Kimihiko AU - Mitsutomi K FAU - Katsuragi, Isao AU - Katsuragi I FAU - Kakuma, Tetsuya AU - Kakuma T FAU - Sakata, Toshiie AU - Sakata T FAU - Yoshimatsu, Hironobu AU - Yoshimatsu H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130319 PL - England TA - J Neurochem JT - Journal of neurochemistry JID - 2985190R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Receptors, Histamine H1) RN - 820484N8I3 (Histamine) RN - 9015-71-8 (Corticotropin-Releasing Hormone) SB - IM MH - Animals MH - Anorexia/chemically induced/*physiopathology MH - Body Weight/drug effects/physiology MH - Brain-Derived Neurotrophic Factor/metabolism/pharmacology/*physiology MH - Corticotropin-Releasing Hormone/*physiology MH - Eating/drug effects/physiology MH - Feeding Behavior/drug effects/*physiology MH - Histamine/metabolism/*physiology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, Histamine H1/genetics/metabolism MH - Ventromedial Hypothalamic Nucleus/drug effects/*physiology EDAT- 2013/02/26 06:00 MHDA- 2013/07/03 06:00 CRDT- 2013/02/26 06:00 PHST- 2012/11/05 00:00 [received] PHST- 2013/02/19 00:00 [revised] PHST- 2013/02/19 00:00 [accepted] PHST- 2013/02/26 06:00 [entrez] PHST- 2013/02/26 06:00 [pubmed] PHST- 2013/07/03 06:00 [medline] AID - 10.1111/jnc.12213 [doi] PST - ppublish SO - J Neurochem. 2013 May;125(4):588-98. doi: 10.1111/jnc.12213. Epub 2013 Mar 19.