PMID- 23433206
OWN - NLM
STAT- MEDLINE
DCOM- 20140109
LR  - 20130225
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 31
IP  - 2
DP  - 2013 Feb
TI  - [Effects of carbon disulfide exposure during peri-implantation on estrogen 
      receptor-alpha expression in uterus and serum level of estrogen in pregnant mice].
PG  - 88-91
AB  - OBJECTIVE: To evaluate the effects of carbon disulfide (CS(2)) exposure during 
      peri-implantation on the estrogen receptor-alpha (ER-alpha) expression in the uterus and 
      serum level of estradiol (E(2)) in pregnant mice, and to explore the mechanism of 
      embryotoxicity of CS(2). METHODS: Healthy female mice were exposed to a single 
      dose of CS(2) (631.4 mg/kg) or olive oil (solvent control) on gestational day 
      (GD)3, GD4, GD5, or GD6. At different time points after exposure, the serum E(2) 
      levels of the pregnant mice were measured by enzyme-linked immunosorbent assay, 
      and the expression levels of ER-alpha in the uterus were measured by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis and Western blot. RESULTS: Compared 
      with the control group, the GD3, GD4, GD5, and GD6 exposure groups showed 
      significantly decreased serum E(2) levels on day 7 of gestation (P < 0.05); the 
      GD3 and GD5 exposure groups showed significantly decreased serum E(2) levels on 
      day 6 of gestation (P < 0.05). The expression level of ER-alpha in the GD 4 exposure 
      group was 23.6% lower than that in the control group on day 5 of gestation, and 
      the expression level of ER-alpha in the GD 5 exposure group was 72.9% lower than that 
      in the control group on day 6 of gestation (P < 0.05); the GD 3 and GD 6 exposure 
      groups showed lower expression levels of ER-alpha than the control group at any time 
      point, but no significant difference was found (P > 0.05). CONCLUSION: CS(2) 
      exposure during peri-implantation can reduce the ER-alpha expression in the uterus 
      and the serum level of E(2) in pregnant mice, which may be one of the mechanisms 
      of embryotoxicity of CS(2).
FAU - Zhang, Bing-zhen
AU  - Zhang BZ
AD  - Institute of Epidemiology and Health Statistics, School of Public Health, 
      Shandong University, Jinan 250012, China.
FAU - Wu, Yan-ling
AU  - Wu YL
FAU - Dai, Bing-qin
AU  - Dai BQ
FAU - Li, Chun-hui
AU  - Li CH
FAU - Yang, Liu
AU  - Yang L
FAU - Wang, Zhi-ping
AU  - Wang ZP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (Estrogen Receptor alpha)
RN  - 4TI98Z838E (Estradiol)
RN  - S54S8B99E8 (Carbon Disulfide)
SB  - IM
MH  - Animals
MH  - Carbon Disulfide/*toxicity
MH  - *Embryo Implantation
MH  - Estradiol/*blood
MH  - Estrogen Receptor alpha/*metabolism
MH  - Female
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Pregnancy
MH  - Uterus/drug effects/*metabolism
EDAT- 2013/02/26 06:00
MHDA- 2014/01/10 06:00
CRDT- 2013/02/26 06:00
PHST- 2013/02/26 06:00 [entrez]
PHST- 2013/02/26 06:00 [pubmed]
PHST- 2014/01/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Feb;31(2):88-91.