PMID- 23435238
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20211021
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 5
IP  - 2
DP  - 2013 Feb 11
TI  - Predicted peptides from non-structural proteins of porcine reproductive and 
      respiratory syndrome virus are able to induce IFN-gamma and IL-10.
PG  - 663-77
LID - 10.3390/v5020663 [doi]
AB  - This work describes peptides from non-structural proteins (nsp) of porcine 
      reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell 
      epitopes by bioinfornatics and tested for their ability to induce IFN-gamma and IL-10 
      responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated 
      vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the 
      peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs 
      were initially screened for IFN-gamma responses (ELISPOT) and three peptides were 
      identified; two of them in non-conserved segments of nsp2 and nsp5 and the other 
      in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 
      inducing properties. Six peptides were found to induce IL-10 release in PBMC and 
      some of them were also able to inhibit IFN-gamma responses on PHA-stimulated cells. 
      Interestingly, the IFN-gamma low responder pigs against PRRSV were mostly homozygous 
      for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV 
      contain T-cell epitopes inducing IFN-gamma responses as well as IL-10 inducing 
      segments with inhibitory capabilities.
FAU - Burgara-Estrella, Alexel
AU  - Burgara-Estrella A
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo 
      A.C (CIAD), Carretera a la Victoria Km 0.6, C.P. 83304 Hermosillo, Sonora, 
      Mexico. alexel_25@hotmail.com
FAU - Diaz, Ivan
AU  - Diaz I
FAU - Rodriguez-Gomez, Irene M
AU  - Rodriguez-Gomez IM
FAU - Essler, Sabine E
AU  - Essler SE
FAU - Hernandez, Jesus
AU  - Hernandez J
FAU - Mateu, Enric
AU  - Mateu E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130211
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (swine leukocyte antigen)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Computational Biology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Haplotypes
MH  - Histocompatibility Antigens Class I
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-10/*biosynthesis
MH  - Leukocytes, Mononuclear/immunology/metabolism/virology
MH  - Peptides/chemistry/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Nonstructural Proteins/*chemistry/*immunology
PMC - PMC3640520
EDAT- 2013/02/26 06:00
MHDA- 2013/09/14 06:00
PMCR- 2013/02/01
CRDT- 2013/02/26 06:00
PHST- 2012/12/26 00:00 [received]
PHST- 2013/02/07 00:00 [revised]
PHST- 2013/02/09 00:00 [accepted]
PHST- 2013/02/26 06:00 [entrez]
PHST- 2013/02/26 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - v5020663 [pii]
AID - viruses-05-00663 [pii]
AID - 10.3390/v5020663 [doi]
PST - epublish
SO  - Viruses. 2013 Feb 11;5(2):663-77. doi: 10.3390/v5020663.