PMID- 23436422
OWN - NLM
STAT- MEDLINE
DCOM- 20130807
LR  - 20151119
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 986
DP  - 2013
TI  - Bright-field in situ hybridization methods to discover gene amplifications and 
      rearrangements in clinical samples.
PG  - 341-52
LID - 10.1007/978-1-62703-311-4_21 [doi]
AB  - Brightfield in situ hybridization (BISH) applications have significant advantages 
      over traditional fluorescence in situ hybridization (FISH). BISH slides can be 
      analyzed using a regular microscope while FISH slides require the use of a 
      specialized fluorescence microscope. BISH slides allow observers for correlating 
      the gene status (gene amplifications, gene rearrangements, and gene deletions) 
      and tissue morphology better than FISH slides. Also, BISH slides are ideal for 
      the permanent preservation of gene signals. Furthermore, BISH applications can be 
      optimized using an automated tissue slide processing system. BISH applications 
      are becoming a popular method for clinical examination of gene status for 
      selecting cancer treatments.
FAU - Nitta, Hiroaki
AU  - Nitta H
AD  - Medical Innovation, Ventana Medical Systems, Inc., Tucson, AZ, USA.
FAU - Grogan, Thomas M
AU  - Grogan TM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies, Monoclonal, Humanized)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - P188ANX8CK (Trastuzumab)
SB  - IM
MH  - Antibodies, Monoclonal, Humanized/therapeutic use
MH  - Breast Neoplasms/drug therapy/genetics
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Gene Rearrangement/*genetics
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Receptor, ErbB-2/analysis
MH  - Recombination, Genetic/genetics
MH  - Trastuzumab
EDAT- 2013/02/26 06:00
MHDA- 2013/08/08 06:00
CRDT- 2013/02/26 06:00
PHST- 2013/02/26 06:00 [entrez]
PHST- 2013/02/26 06:00 [pubmed]
PHST- 2013/08/08 06:00 [medline]
AID - 10.1007/978-1-62703-311-4_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;986:341-52. doi: 10.1007/978-1-62703-311-4_21.