PMID- 23436435
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20220309
IS  - 1098-1063 (Electronic)
IS  - 1050-9631 (Linking)
VI  - 23
IP  - 5
DP  - 2013 May
TI  - Regulation of the spatial code for BDNF mRNA isoforms in the rat hippocampus 
      following pilocarpine-treatment: a systematic analysis using laser 
      microdissection and quantitative real-time PCR.
PG  - 413-23
LID - 10.1002/hipo.22100 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is essential for neuronal survival, 
      differentiation, and plasticity and is one of those genes that generate multiple 
      mRNAs with different alternatively spliced 5'UTRs. The functional significance of 
      many BDNF transcripts, each producing the same protein, is emerging. On the basis 
      of the analysis of the four most abundant brain BDNF transcripts, we recently 
      proposed the "spatial code hypothesis of BDNF splice variants" according to which 
      the BDNF transcripts, through their differential subcellular localization in soma 
      or dendrites, represent a mechanism to synthesize the protein at distinct 
      locations and produce local effects. In this study, using laser microdissection 
      of hippocampal laminae and reverse transcription-quantitative real-time PCR 
      (RT-qPCR), we analyzed all known BDNF mRNA variants at resting conditions or 
      following 3 h pilocarpine-induced status epilepticus. In untreated rats, we found 
      dendritic enrichment of BDNF transcripts encoding exons 6 and 7 in CA1; exons 1, 
      6, and 9a in CA3; and exons 5, 6, 7, and 8 in DG. Considering the low abundance 
      of the other transcripts, exon 6 was the main transcript in dendrites under 
      resting conditions. Pilocarpine treatment induced an increase of BDNF transcripts 
      encoding exons 4 and 6 in all dendritic laminae and, additionally, of exon 2 in 
      CA1 stratum radiatum and exons 2, 3, 9a in DG molecular layer while the other 
      transcripts were decreased in dendrites, suggesting restriction to the soma. 
      These results support the hypothesis of a spatial code to differentially regulate 
      BDNF in the somatic or dendritic compartment under conditions of 
      pilocarpine-induced status epilepticus and, furthermore, highlight the existence 
      of subfield-specific differences.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Baj, Gabriele
AU  - Baj G
AD  - Department of Life Science, B.R.A.I.N. Center for Neuroscience, University of 
      Trieste, Trieste, Italy.
FAU - Del Turco, Domenico
AU  - Del Turco D
FAU - Schlaudraff, Jessica
AU  - Schlaudraff J
FAU - Torelli, Lucio
AU  - Torelli L
FAU - Deller, Thomas
AU  - Deller T
FAU - Tongiorgi, Enrico
AU  - Tongiorgi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130225
PL  - United States
TA  - Hippocampus
JT  - Hippocampus
JID - 9108167
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Muscarinic Agonists)
RN  - 0 (RNA, Messenger)
RN  - 01MI4Q9DI3 (Pilocarpine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Dendrites/drug effects/metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Hippocampus/cytology/*drug effects/metabolism
MH  - Male
MH  - Microdissection
MH  - Muscarinic Agonists/*pharmacology
MH  - Neurons/cytology/drug effects
MH  - Pilocarpine/*pharmacology
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Statistics, Nonparametric
EDAT- 2013/02/26 06:00
MHDA- 2013/10/01 06:00
CRDT- 2013/02/26 06:00
PHST- 2013/01/08 00:00 [accepted]
PHST- 2013/02/26 06:00 [entrez]
PHST- 2013/02/26 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
AID - 10.1002/hipo.22100 [doi]
PST - ppublish
SO  - Hippocampus. 2013 May;23(5):413-23. doi: 10.1002/hipo.22100. Epub 2013 Feb 25.