PMID- 23436553
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20151119
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 24
IP  - 4
DP  - 2013 Jul-Aug
TI  - Determination of endogenous brassinosteroids in plant tissues using solid-phase 
      extraction with double layered cartridge followed by high-performance liquid 
      chromatography-tandem mass spectrometry.
PG  - 386-94
LID - 10.1002/pca.2421 [doi]
AB  - INTRODUCTION: Brassinosteroids (BRs) are a group of important phytohormones that 
      play vital roles in plant growth, development and a series of physiological 
      phenomena. In order to understand biosynthesis, degradation and metabolic 
      pathways of BRs, a reliable analytical method of BRs with effective sample 
      pre-treatment process is favourable. OBJECTIVE: The development of a quick and 
      effective method for the quantification of endogenous BRs in plant tissue with 
      the aid of double layered solid-phase extraction (SPE) cartridges (graphite 
      carbon black and primary secondary amine silica sorbent: GCB/PSA). METHOD: The 
      method involved an initial extraction of BRs with acetonitrile, a dehydration 
      process with anhydrous MgSO4 and NaCl, a SPE purification process with a double 
      layered cartridge, and a further clean-up step utilising liquid-liquid extraction 
      (LLE). The purification process was mainly realised on the GCB/PSA cartridge. GCB 
      could eliminate hydrophobic compounds, especially those containing a pi system, 
      and PSA was introduced to remove the polar interferences. Endogenous BRs were 
      quantified by HPLC-ESI-MS/MS. RESULTS: Good linearities were obtained in the 
      range of 0.4-500 ng/mL (0.0124-15 ng), with the correlation coefficients above 
      0.9957. The relative recoveries of BRs of this method were in the range of 
      71.1-113.1%, with intra- and interday relative standard deviations (RSDs) less 
      than 16.3%. With the proposed method, the requirement of plant tissue amount was 
      minimised to 1 g fresh weight, which is the smallest amount reported so far, to 
      our knowledge.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Ding, Jun
AU  - Ding J
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, 
      China.
FAU - Mao, Li-Jing
AU  - Mao LJ
FAU - Wang, Shao-Ting
AU  - Wang ST
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130225
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Acetonitriles)
RN  - 0 (Brassinosteroids)
RN  - 0 (Buffers)
RN  - 0 (Solvents)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Brassica napus/chemistry
MH  - Brassinosteroids/*analysis/isolation & purification
MH  - Buffers
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Liquid-Liquid Extraction
MH  - Oryza/chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/instrumentation/*methods
MH  - Solvents/chemistry
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/02/26 06:00
MHDA- 2014/02/05 06:00
CRDT- 2013/02/26 06:00
PHST- 2012/09/25 00:00 [received]
PHST- 2012/12/17 00:00 [revised]
PHST- 2012/12/26 00:00 [accepted]
PHST- 2013/02/26 06:00 [entrez]
PHST- 2013/02/26 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
AID - 10.1002/pca.2421 [doi]
PST - ppublish
SO  - Phytochem Anal. 2013 Jul-Aug;24(4):386-94. doi: 10.1002/pca.2421. Epub 2013 Feb 
      25.