PMID- 23438035
OWN - NLM
STAT- MEDLINE
DCOM- 20140314
LR  - 20220311
IS  - 1750-3639 (Electronic)
IS  - 1015-6305 (Print)
IS  - 1015-6305 (Linking)
VI  - 23
IP  - 5
DP  - 2013 Sep
TI  - PDGFRA amplification is common in pediatric and adult high-grade astrocytomas and 
      identifies a poor prognostic group in IDH1 mutant glioblastoma.
PG  - 565-73
LID - 10.1111/bpa.12043 [doi]
AB  - High-grade astrocytomas (HGAs), corresponding to World Health Organization grades 
      III (anaplastic astrocytoma) and IV (glioblastoma; GBM), are biologically 
      aggressive, and their molecular classification is increasingly relevant to 
      clinical management. PDGFRA amplification is common in HGAs, although its 
      prognostic significance remains unclear. Using fluorescence in situ hybridization 
      (FISH), the most sensitive technique for detecting PDGFRA copy number gains, we 
      determined PDGFRA amplification status in 123 pediatric and 263 adult HGAs. A 
      range of PDGFRA FISH patterns were identified and cases were scored as 
      non-amplified (normal and polysomy) or amplified (low-level and high-level). 
      PDGFRA amplification was frequent in pediatric (29.3%) and adult (20.9%) tumors. 
      Amplification was not prognostic in pediatric HGAs. In adult tumors diagnosed 
      initially as GBM, the presence of combined PDGFRA amplification and isocitrate 
      dehydrogenase 1 (IDH1)(R132H) mutation was a significant independent prognostic 
      factor (P = 0.01). In HGAs, PDGFRA amplification is common and can manifest as 
      high-level and focal or low-level amplifications. Our data indicate that the 
      latter is more prevalent than previously reported with copy number averaging 
      techniques. To our knowledge, this is the largest survey of PDGFRA status in 
      adult and pediatric HGAs and suggests PDGFRA amplification increases with grade 
      and is associated with a less favorable prognosis in IDH1 mutant de novo GBMs.
CI  - (c) 2013 International Society of Neuropathology.
FAU - Phillips, Joanna J
AU  - Phillips JJ
AD  - Department of Pathology, University of California San Francisco, San Francisco, 
      CA, USA. joanna.phillips@ucsf.edu
FAU - Aranda, Derick
AU  - Aranda D
FAU - Ellison, David W
AU  - Ellison DW
FAU - Judkins, Alexander R
AU  - Judkins AR
FAU - Croul, Sidney E
AU  - Croul SE
FAU - Brat, Daniel J
AU  - Brat DJ
FAU - Ligon, Keith L
AU  - Ligon KL
FAU - Horbinski, Craig
AU  - Horbinski C
FAU - Venneti, Sriram
AU  - Venneti S
FAU - Zadeh, Gelareh
AU  - Zadeh G
FAU - Santi, Mariarita
AU  - Santi M
FAU - Zhou, Shengmei
AU  - Zhou S
FAU - Appin, Christina L
AU  - Appin CL
FAU - Sioletic, Stefano
AU  - Sioletic S
FAU - Sullivan, Lisa M
AU  - Sullivan LM
FAU - Martinez-Lage, Maria
AU  - Martinez-Lage M
FAU - Robinson, Aaron E
AU  - Robinson AE
FAU - Yong, William H
AU  - Yong WH
FAU - Cloughesy, Timothy
AU  - Cloughesy T
FAU - Lai, Albert
AU  - Lai A
FAU - Phillips, Heidi S
AU  - Phillips HS
FAU - Marshall, Roxanne
AU  - Marshall R
FAU - Mueller, Sabine
AU  - Mueller S
FAU - Haas-Kogan, Daphne A
AU  - Haas-Kogan DA
FAU - Molinaro, Annette M
AU  - Molinaro AM
FAU - Perry, Arie
AU  - Perry A
LA  - eng
GR  - K08 NS063456/NS/NINDS NIH HHS/United States
GR  - 1U01CA168878/CA/NCI NIH HHS/United States
GR  - U01 CA168878/CA/NCI NIH HHS/United States
GR  - P01 CA095616/CA/NCI NIH HHS/United States
GR  - P50 CA097257/CA/NCI NIH HHS/United States
GR  - P01CA095616/CA/NCI NIH HHS/United States
GR  - K08 CA155764/CA/NCI NIH HHS/United States
GR  - CA097257/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130318
PL  - Switzerland
TA  - Brain Pathol
JT  - Brain pathology (Zurich, Switzerland)
JID - 9216781
RN  - EC 1.1.1.41 (Isocitrate Dehydrogenase)
RN  - EC 1.1.1.42. (IDH1 protein, human)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Astrocytoma/*genetics
MH  - Brain Neoplasms/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Cohort Studies
MH  - DNA Copy Number Variations/*genetics
MH  - Female
MH  - Glioblastoma/*genetics
MH  - Humans
MH  - Isocitrate Dehydrogenase/*genetics
MH  - Male
MH  - Middle Aged
MH  - Mutation/*genetics
MH  - Receptor, Platelet-Derived Growth Factor alpha/*genetics
MH  - Sex Factors
MH  - Statistics, Nonparametric
MH  - Young Adult
PMC - PMC3715570
MID - NIHMS452642
OTO - NOTNLM
OT  - FISH
OT  - IDH1
OT  - PDGFRA
OT  - astrocytoma
OT  - isocitrate dehydrogenase 1
OT  - prognosis
COIS- The authors declare they have no conflict of interest.
EDAT- 2013/02/27 06:00
MHDA- 2014/03/15 06:00
PMCR- 2013/03/18
CRDT- 2013/02/27 06:00
PHST- 2012/10/24 00:00 [received]
PHST- 2013/02/11 00:00 [accepted]
PHST- 2013/02/27 06:00 [entrez]
PHST- 2013/02/27 06:00 [pubmed]
PHST- 2014/03/15 06:00 [medline]
PHST- 2013/03/18 00:00 [pmc-release]
AID - BPA12043 [pii]
AID - 10.1111/bpa.12043 [doi]
PST - ppublish
SO  - Brain Pathol. 2013 Sep;23(5):565-73. doi: 10.1111/bpa.12043. Epub 2013 Mar 18.