PMID- 23439211
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20211021
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 125
IP  - 4
DP  - 2013 May
TI  - Astrocytes inhibit microglial surface expression of dendritic cell-related 
      co-stimulatory molecules through a contact-mediated process.
PG  - 575-87
LID - 10.1111/jnc.12221 [doi]
AB  - Murine microglia cultured in isolation were treated sequentially with 
      granulocyte/monocyte colony-stimulating factor (GM-CSF) (5 days) and 
      lipopolysaccharide (LPS) (2 days) to elicit a mature dendritic cell-like 
      (DC-like) phenotype. Examined by flow cytometry microglia thus isolated show high 
      surface expression of CD11c together with the co-stimulatory molecules CD40, 
      CD80, and CD86 that are necessary for T-cell activation. In contrast, microglia 
      co-cultured with astrocytes fail to achieve a mature DC-like phenotype. Contact 
      with the astrocytic environment is necessary for the inhibition. Failure was not 
      because of a more rapid degradation of protein. Bone marrow-derived cells, like 
      microglia, were prevented by astrocytes from attaining a mature DC phenotype. 
      Although GM-CSF pre-treatment substantially increases mRNA of co-stimulatory 
      molecules and major histocompatibility complex (MHC) Class II in isolated 
      microglia, co-cultured microglia await treatment with LPS to up-regulate them. In 
      contrast, western blot and immunocytochemical analysis revealed that it is not a 
      failure of transcription or translation, nor is it a more rapid degradation of 
      mRNA that is responsible for the low surface expression; rather microglia 
      co-cultured with astrocytes produce mRNA and protein but do not traffic the 
      protein onto the cell surface.
CI  - (c) 2013 International Society for Neurochemistry.
FAU - Acevedo, Giselles
AU  - Acevedo G
AD  - Rutgers University/Newark, Newark, New Jersey, USA.
FAU - Padala, Nischal K
AU  - Padala NK
FAU - Ni, Li
AU  - Ni L
FAU - Jonakait, G M
AU  - Jonakait GM
LA  - eng
GR  - R25 GM060826/GM/NIGMS NIH HHS/United States
GR  - R25 GM096161/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130327
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antigens, Surface)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RNA, Messenger)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, Surface/genetics/metabolism
MH  - Astrocytes/*cytology/physiology
MH  - B7-1 Antigen/*genetics/metabolism
MH  - B7-2 Antigen/*genetics/metabolism
MH  - CD11c Antigen/genetics/metabolism
MH  - CD40 Antigens/*genetics/metabolism
MH  - Cell Communication/physiology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*cytology/physiology
MH  - Female
MH  - Gene Expression/drug effects/physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/*cytology/physiology
MH  - Phenotype
MH  - RNA, Messenger/metabolism
PMC - PMC3637427
MID - NIHMS449585
COIS- The authors have no conflicts of interest to declare.
EDAT- 2013/02/27 06:00
MHDA- 2013/07/03 06:00
PMCR- 2014/05/01
CRDT- 2013/02/27 06:00
PHST- 2013/01/20 00:00 [received]
PHST- 2013/02/08 00:00 [revised]
PHST- 2013/02/08 00:00 [accepted]
PHST- 2013/02/27 06:00 [entrez]
PHST- 2013/02/27 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - 10.1111/jnc.12221 [doi]
PST - ppublish
SO  - J Neurochem. 2013 May;125(4):575-87. doi: 10.1111/jnc.12221. Epub 2013 Mar 27.