PMID- 23441686
OWN - NLM
STAT- MEDLINE
DCOM- 20130813
LR  - 20231104
IS  - 1520-6025 (Electronic)
IS  - 0163-3864 (Print)
IS  - 0163-3864 (Linking)
VI  - 76
IP  - 4
DP  - 2013 Apr 26
TI  - Comparative study of chromatographic medium-associated mass and potential 
      antitumor activity loss with bioactive extracts.
PG  - 642-7
LID - 10.1021/np300858c [doi]
AB  - Natural product drug discovery programs often rely on the use of silica (Si) gel, 
      reversed-phase media, or size-exclusion resins (e.g., RP-C18, Sephadex LH-20) for 
      compound purification. The synthetic polymer-based sorbent Diaion HP20SS 
      (cross-linked polystyrene matrix) is used as an alternative to prepare purified 
      natural product libraries. To evaluate the impact of chromatographic media on the 
      isolation of biologically active, yet chromatographically unstable natural 
      products, Diaion HP20SS was evaluated side-by-side with normal-phase sorbents for 
      irreversible binding of extract constituents and their effects on bioactivity. An 
      array of chemically diverse natural product-rich extracts was selected as a test 
      panel, and a cell-based reporter assay for hypoxia-inducible factor-1 (HIF-1) was 
      employed to monitor potential change(s) in bioactivity. Silica gel caused 
      significant irreversible binding of three out of 10 extracts. Curcuma longa, 
      Saururus cernuus, and Citrus reticulata extracts showed decreased HIF-1 
      inhibitory activity after elution through Si gel. An additional nonpolar column 
      wash of HP20SS with EtOAc retained considerable bioactivities of active extracts. 
      In general, Si gel produced the greatest loss of bioactivity. However, HP20SS 
      elution reduced significantly HIF-1 inhibitory activity of certain extracts 
      (e.g., Asimina triloba).
FAU - Datta, Sandipan
AU  - Datta S
AD  - Department of Pharmacognosy, School of Pharmacy, University of Mississippi, 
      Mississippi 38677, United States.
FAU - Zhou, Yu-Dong
AU  - Zhou YD
FAU - Nagle, Dale G
AU  - Nagle DG
LA  - eng
GR  - C06 RR-14503/RR/NCRR NIH HHS/United States
GR  - C06 RR014503/RR/NCRR NIH HHS/United States
GR  - CA98787/CA/NCI NIH HHS/United States
GR  - R01 CA098787/CA/NCI NIH HHS/United States
GR  - R56 CA098787/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130226
PL  - United States
TA  - J Nat Prod
JT  - Journal of natural products
JID - 7906882
RN  - 0 (Biological Products)
RN  - 0 (Dextrans)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Plant Extracts)
RN  - 60650-90-0 (Silica Gel)
RN  - 9014-76-0 (sephadex)
SB  - IM
MH  - Asimina/chemistry
MH  - Aspalathus/chemistry
MH  - Biological Products
MH  - Chromatography
MH  - Citrus/chemistry
MH  - Curcuma/chemistry
MH  - Cyclopia Plant/chemistry
MH  - Dextrans
MH  - Female
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*antagonists & inhibitors
MH  - Lythraceae/chemistry
MH  - Plant Extracts/analysis/*chemistry
MH  - Podophyllum/*chemistry
MH  - Saururaceae/chemistry
MH  - Silica Gel
MH  - Vaccinium macrocarpon/chemistry
PMC - PMC3683388
MID - NIHMS449870
COIS- The authors declare no competing financial interests.
EDAT- 2013/02/28 06:00
MHDA- 2013/08/14 06:00
PMCR- 2014/04/26
CRDT- 2013/02/28 06:00
PHST- 2013/02/28 06:00 [entrez]
PHST- 2013/02/28 06:00 [pubmed]
PHST- 2013/08/14 06:00 [medline]
PHST- 2014/04/26 00:00 [pmc-release]
AID - 10.1021/np300858c [doi]
PST - ppublish
SO  - J Nat Prod. 2013 Apr 26;76(4):642-7. doi: 10.1021/np300858c. Epub 2013 Feb 26.