PMID- 23441819
OWN - NLM
STAT- MEDLINE
DCOM- 20140725
LR  - 20220321
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 46
IP  - 8
DP  - 2013 Aug
TI  - Antibiofilm activity, pH and solubility of endodontic sealers.
PG  - 755-62
LID - 10.1111/iej.12055 [doi]
AB  - AIM: To evaluate antibiofilm activity against Enterococcus faecalis, pH and 
      solubility of AH Plus, Sealer 26, Epiphany SE, Sealapex, Activ GP, MTA Fillapex 
      (MTA-F) and an experimental MTA-based Sealer (MTA-S). METHODOLOGY: Sealer samples 
      were manipulated and stored for 2 or 7 days. Prepared sealers were evaluated by a 
      modified direct contact test (DCT) for 5 h, 10 h or 15 h with biofilm previously 
      induced on bovine dentine for 14 days. In the control group, the biofilm was not 
      exposed to the sealers. The number of colony-forming units (CFU mL(-1) ) in the 
      remaining biofilm was determined. Sealer solubility was assessed by the 
      percentage of mass loss after 15 h of immersion in distilled water. Sealer pH was 
      measured at 5 h, 10 h and 15 h. Statistical analysis was performed using 
      Kruskal-Wallis and Dunn or anova and Tamhane's T2 tests, at 5% significance. 
      RESULTS: At 2 days post-manipulation, the DCT showed that Sealapex and MTA-F were 
      associated with a reduction in the number of bacteria in all 3 contact periods 
      evaluated, compared with the control group (P < 0.05). At 7 days, Sealapex had 
      the greatest antibiofilm action at 10 h and 15 h. Sealapex had the highest pH 
      values 2 and 7 days post-manipulation. Regarding the solubility, at 2 days the 
      highest values were observed for MTA-F, MTA-S, Sealapex and Activ GP (P < 0.05). 
      At 7 days, MTA-S and MTA-F had greater solubility than the other materials 
      (P < 0.05). AH Plus had the lowest solubility for both post-manipulation periods 
      (P < 0.05). CONCLUSION: Sealapex and MTA-F were associated with a reduction in 
      the number of bacteria in biofilms and had greater solubility. The high 
      solubility and pH may be related to the antibacterial activity of these 
      materials.
CI  - (c) 2012 International Endodontic Journal. Published by John Wiley & Sons Ltd.
FAU - Faria-Junior, N B
AU  - Faria-Junior NB
AD  - Department of Restorative Dentistry, Araraquara Dental School, Sao Paulo State 
      University, Araraquara, Brazil.
FAU - Tanomaru-Filho, M
AU  - Tanomaru-Filho M
FAU - Berbert, F L C V
AU  - Berbert FL
FAU - Guerreiro-Tanomaru, J M
AU  - Guerreiro-Tanomaru JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130226
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Acrylic Resins)
RN  - 0 (Aluminum Compounds)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Calcium Compounds)
RN  - 0 (Drug Combinations)
RN  - 0 (Epoxy Resins)
RN  - 0 (Oxides)
RN  - 0 (Root Canal Filling Materials)
RN  - 0 (Salicylates)
RN  - 0 (Sealer 26)
RN  - 0 (Silicates)
RN  - 0 (activ GP sealer)
RN  - 0 (epiphany sealer)
RN  - 0 (epoxy resin-based root canal sealer)
RN  - 0 (mineral trioxide aggregate)
RN  - 059QF0KO0R (Water)
RN  - 115055-59-9 (Sealapex)
RN  - PF5DZW74VN (Calcium Hydroxide)
RN  - U015TT5I8H (Bismuth)
MH  - Acrylic Resins/chemistry/pharmacology
MH  - Aluminum Compounds/chemistry/pharmacology
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Load/drug effects
MH  - Biofilms/drug effects
MH  - Bismuth/chemistry/pharmacology
MH  - Calcium Compounds/chemistry/pharmacology
MH  - Calcium Hydroxide/chemistry/pharmacology
MH  - Cattle
MH  - Dentin/microbiology
MH  - Drug Combinations
MH  - Enterococcus faecalis/drug effects/physiology
MH  - Epoxy Resins/chemistry/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Materials Testing
MH  - Oxides/chemistry/pharmacology
MH  - Root Canal Filling Materials/chemistry/*pharmacology
MH  - Salicylates/chemistry/pharmacology
MH  - Silicates/chemistry/pharmacology
MH  - Solubility
MH  - Time Factors
MH  - Water/chemistry
OTO - NOTNLM
OT  - antibacterial activity
OT  - endodontic sealers
OT  - pH
OT  - solubility
EDAT- 2013/02/28 06:00
MHDA- 2014/07/26 06:00
CRDT- 2013/02/28 06:00
PHST- 2012/07/02 00:00 [received]
PHST- 2012/12/14 00:00 [accepted]
PHST- 2013/02/28 06:00 [entrez]
PHST- 2013/02/28 06:00 [pubmed]
PHST- 2014/07/26 06:00 [medline]
AID - 10.1111/iej.12055 [doi]
PST - ppublish
SO  - Int Endod J. 2013 Aug;46(8):755-62. doi: 10.1111/iej.12055. Epub 2013 Feb 26.