PMID- 23442576
OWN - NLM
STAT- MEDLINE
DCOM- 20131122
LR  - 20211021
IS  - 1554-8635 (Electronic)
IS  - 1554-8627 (Print)
IS  - 1554-8627 (Linking)
VI  - 9
IP  - 5
DP  - 2013 May
TI  - Viroporin-mediated calcium-activated autophagy.
PG  - 797-8
LID - 10.4161/auto.23959 [doi]
AB  - Autophagy is a cellular response activated by many pathogens, but the mechanism 
      of activation is largely unknown. Recently we showed for the first time that 
      rotavirus initiates the autophagy pathway through a calcium-mediated mechanism. 
      Expression of the rotavirus-encoded NSP4, a pore-forming protein (viroporin), 
      elicits the release of endoplasmic reticulum (ER) lumenal calcium into the 
      cytoplasm of the infected cell. The increased cytoplasmic calcium activates a 
      calcium signaling pathway involving calcium/calmodulin-dependent protein kinase 
      kinase 2 (CAMKK2) and 5' adenosine monophosphate-activated protein kinase (AMPK) 
      to trigger autophagy. Rotavirus further manipulates autophagy membrane 
      trafficking to transport viral ER-associated proteins to viroplasms, sites of 
      viral genome replication and immature particle assembly. Transport of viral 
      proteins to viroplasms is required for assembly of infectious virus. Thus, NSP4, 
      a multifunctional viral protein known to regulate infectious particle assembly, 
      also modulates membrane trafficking by orchestrating the activation of autophagy 
      to benefit viral replication.
FAU - Crawford, Sue E
AU  - Crawford SE
AD  - Department of Molecular Virology and Microbiology, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Estes, Mary K
AU  - Estes MK
LA  - eng
GR  - P30 DK056338/DK/NIDDK NIH HHS/United States
GR  - R01 AI080656/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20130226
PL  - United States
TA  - Autophagy
JT  - Autophagy
JID - 101265188
RN  - 0 (Glycoproteins)
RN  - 0 (NS28 protein, rotavirus)
RN  - 0 (Toxins, Biological)
RN  - 0 (Viral Nonstructural Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CON - Crawford SE, Hyser JM, Utama B, Estes MK. Autophagy hijacked through 
      viroporin-activated calcium/calmodulin-dependent kinase kinase-beta signaling is 
      required for rotavirus replication. Proc Natl Acad Sci U S A. 2012;109:E3405-13. 
      doi: 10.1073/pnas.1216539109.
MH  - Animals
MH  - Autophagy/drug effects
MH  - Calcium/*pharmacology
MH  - Endoplasmic Reticulum/drug effects/metabolism
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Models, Biological
MH  - Rotavirus/drug effects/physiology
MH  - Signal Transduction/*drug effects
MH  - Toxins, Biological/*metabolism
MH  - Viral Nonstructural Proteins/*metabolism
MH  - Virus Assembly/drug effects
PMC - PMC3669193
OTO - NOTNLM
OT  - autophagy
OT  - calcium
OT  - kinase
OT  - stress
OT  - virus
EDAT- 2013/02/28 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/05/01
CRDT- 2013/02/28 06:00
PHST- 2013/02/28 06:00 [entrez]
PHST- 2013/02/28 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - 23959 [pii]
AID - 2013AUTO0104 [pii]
AID - 10.4161/auto.23959 [doi]
PST - ppublish
SO  - Autophagy. 2013 May;9(5):797-8. doi: 10.4161/auto.23959. Epub 2013 Feb 26.