PMID- 23443577
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20130301
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 31
IP  - 4
DP  - 2013 Apr
TI  - Expression profiles of miRNAs in polarized macrophages.
PG  - 797-802
LID - 10.3892/ijmm.2013.1260 [doi]
AB  - microRNAs (miRNAs) play a crucial role in tissue development and the pathology of 
      various diseases. However, the effects and roles of miRNAs in macrophage 
      polarization have yet to be investigated. In this study, we analyzed and compared 
      the miRNA expression profiles of bone marrow-derived macrophages (BMDMs) with two 
      distinct polarizing conditions (classical macrophage activation 'M1' and 
      alternative activation 'M2') using miRNA microarray. In total, 109 miRNAs were 
      differentially expressed between M1 and M2. The differential expression of 
      selected miRNAs was validated by real-time qRT-PCR: miR-181a, miR-155-5p, 
      miR-204-5p and miR-451 were upregulated (fold change >2, P<0.05) and miR-125-5p, 
      miR-146a-3p, miR-143-3p and miR-145-5p were downregulated (fold change <-2, 
      P<0.05) in M1 compared with M2. In conclusion, our study may be useful for 
      exploring the precise roles of miRNAs in macrophage differentiation and polarized 
      activation processes in the future.
FAU - Zhang, Yingying
AU  - Zhang Y
AD  - Laboratory Medicine of Yijishan Hospital, Wuhu, Anhui 241001, PR China.
FAU - Zhang, Mengying
AU  - Zhang M
FAU - Zhong, Min
AU  - Zhong M
FAU - Suo, Qifeng
AU  - Suo Q
FAU - Lv, Kun
AU  - Lv K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130130
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Antigens, CD)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MicroRNAs)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Bone Marrow Cells
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Interferon-gamma/metabolism
MH  - Lipopolysaccharides/immunology
MH  - Macrophage Activation/*physiology
MH  - Macrophages/chemistry/*metabolism
MH  - Mice
MH  - MicroRNAs/analysis/genetics/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
EDAT- 2013/02/28 06:00
MHDA- 2014/04/25 06:00
CRDT- 2013/02/28 06:00
PHST- 2012/10/30 00:00 [received]
PHST- 2012/12/21 00:00 [accepted]
PHST- 2013/02/28 06:00 [entrez]
PHST- 2013/02/28 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
AID - 10.3892/ijmm.2013.1260 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 Apr;31(4):797-802. doi: 10.3892/ijmm.2013.1260. Epub 2013 Jan 
      30.