PMID- 2344394
OWN - NLM
STAT- MEDLINE
DCOM- 19900702
LR  - 20071114
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 9
IP  - 2
DP  - 1990 Mar
TI  - Isolation of a genomic sublibrary enriched for glucocorticoid-regulated genes.
PG  - 95-102
AB  - Glucocorticoids regulate gene expression by causing the glucocorticoid receptor 
      to bind to an enhancer-like DNA element termed the glucocorticoid regulatory 
      element (GRE). The resultant effect on transcription of specific genes causes a 
      cascade of intracellular events that determines the growth or differentiated 
      function of the target tissue. Although virtually all animal tissues respond to 
      glucocorticoids, it has proven difficult to elucidate the molecular events which 
      underlie physiologically important glucocorticoid effects such as lymphocyte 
      death or poor wound healing. In this paper, a tryptic fragment of the 
      glucocorticoid receptor (17K-GR) is shown to bind selectively to DNA containing a 
      GRE. When a mixture of the mouse mammary tumor virus (MMTV) long terminal repeat 
      (LTR) region and plasmid vector DNA was extracted using the intact glucocorticoid 
      receptor or the 17K-GR, the 17K-GR retained a greater proportion of LTR vs. 
      plasmid DNA. The 17K-GR-LTR complex was also more resistant to salt extraction. 
      Extraction of Bam HI-digested mouse genomic DNA resulted in enrichment of the 
      pro-opiomelanocortin (POMC) gene 5' fragment (which contains a GRE) vs. the 3' 
      fragment which does not. A mouse genomic phage library was enriched for 
      GRE-containing sequences by extraction using the 17K-GR. The frequency of 
      POMC-positive plaques was determined to gauge enrichment of down-regulated genes, 
      and the frequency of phosphoenolpyruvate carboxy-kinase-positive plaques was 
      determined to gauge enrichment of up-regulated genes. The frequencies obtained 
      (1.2 x 10(-3) and 3.5 x 10(-3), respectively) indicated that a family of 
      glucocorticoid-regulated genes totaling approximately 300 had been isolated in a 
      genomic sublibrary.
FAU - Harrison, R W 3rd
AU  - Harrison RW 3rd
AD  - Division of Endocrinology/Metabolism, University of Arkansas for Medical 
      Sciences, Little Rock 72205.
FAU - Lippman, S S
AU  - Lippman SS
FAU - Hendry, W J 3rd
AU  - Hendry WJ 3rd
FAU - Chien, M C
AU  - Chien MC
LA  - eng
GR  - AM 32877/AM/NIADDK NIH HHS/United States
GR  - CA 42091/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (DNA, Recombinant)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glucocorticoids)
RN  - 0 (Receptors, Glucocorticoid)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Recombinant/*isolation & purification
MH  - DNA-Binding Proteins/*physiology
MH  - Genomic Library
MH  - Glucocorticoids/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, Glucocorticoid/*physiology
MH  - *Regulatory Sequences, Nucleic Acid
EDAT- 1990/03/01 00:00
MHDA- 1990/03/01 00:01
CRDT- 1990/03/01 00:00
PHST- 1990/03/01 00:00 [pubmed]
PHST- 1990/03/01 00:01 [medline]
PHST- 1990/03/01 00:00 [entrez]
AID - 10.1089/dna.1990.9.95 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1990 Mar;9(2):95-102. doi: 10.1089/dna.1990.9.95.