PMID- 23447104
OWN - NLM
STAT- MEDLINE
DCOM- 20160201
LR  - 20150422
IS  - 1752-8976 (Electronic)
IS  - 1470-3203 (Linking)
VI  - 16
IP  - 1
DP  - 2015 Mar
TI  - Genetic combination of angiotensin-converting enzyme with methylene 
      tetrahydrofolate reductase polymorphisms and the risk of type 2 diabetes mellitus 
      in Bahrain.
PG  - 172-7
LID - 10.1177/1470320313478286 [doi]
AB  - INTRODUCTION: Bahrain has a high prevalence of type 2 diabetes mellitus (T2DM). 
      Previously, Angiotensin-converting enzyme (ACE) insertion/deletion (I/D) 
      polymorphism was found to be associated with T2DM in Bahrainis. The relationship 
      between the disease progression in Bahraini T2DM population and the genetic 
      polymorphism of methylene-tetrahydrofolate-reductase (MTHFR) C677T is still under 
      investigation. AIM: The current study investigated the distribution of MTHFR 
      C677T gene polymorphism among Bahraini T2DM patients and examined the interaction 
      between ACE I/D and MTHFR C677T polymorphisms on the risk of developing T2DM and 
      its long-term complications. MATERIALS AND METHODS: Polymerase chain reaction 
      (PCR) and PCR-restriction fragment length polymorphism (RFLP) were used to test 
      for the presence of ACE I/D and MTHFR C677T polymorphisms in 171 patients with 
      T2DM compared to 188 healthy (non-diabetic) age-matched control subjects from 
      Bahrain. RESULTS: The incidence of the DD genotype and D allele of the ACE gene 
      was high among Bahraini T2DM patients. MTHFR allele and genotype frequencies did 
      not differ between patients and controls. No significant relationship was 
      identified between the combinations of ACE I/D and MTHFR C677T polymorphisms with 
      T2DM. CONCLUSIONS: The results clearly showed an association of the ACE I/D 
      polymorphism with the progression of T2DM, but when it interacts with MTHFR 
      polymorphism no influence was detected on the increased risk of T2DM.
CI  - (c) The Author(s) 2013.
FAU - Al-Harbi, Einas M
AU  - Al-Harbi EM
AD  - Molecular Genetics Laboratory, Kuwait Medical Genetic Center, State of Kuwait 
      alharbi_em@yahoo.com.
FAU - Farid, Eman M
AU  - Farid EM
AD  - College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of 
      Bahrain.
FAU - Gumaa, Khalid A
AU  - Gumaa KA
AD  - College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of 
      Bahrain.
FAU - Darwish, Abdulla H
AU  - Darwish AH
AD  - Department of Pathology, Bahrain Defense Force Royal Medical Services Hospital, 
      Kingdom of Bahrain.
FAU - Alenizi, Mohammad
AU  - Alenizi M
AD  - General Department of Criminal Evidences, Ministry of Interior, Farwaneyia, State 
      of Kuwait.
FAU - Singh, Jaipaul
AU  - Singh J
AD  - School of Forensic and Investigative Sciences, University of Central Lancashire, 
      UK.
LA  - eng
PT  - Journal Article
DEP - 20130227
PL  - England
TA  - J Renin Angiotensin Aldosterone Syst
JT  - Journal of the renin-angiotensin-aldosterone system : JRAAS
JID - 100971636
RN  - EC 1.5.1.20 (MTHFR protein, human)
RN  - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
RN  - EC 3.4.15.1 (ACE protein, human)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bahrain/epidemiology
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*epidemiology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
MH  - Middle Aged
MH  - Peptidyl-Dipeptidase A/*genetics
MH  - Polymorphism, Genetic/genetics
OTO - NOTNLM
OT  - Bahrain
OT  - angiotensin-converting enzyme
OT  - methylene-tetrahydrofolate-reductase
OT  - polymorphism
OT  - type 2 diabetes mellitus
EDAT- 2013/03/01 06:00
MHDA- 2016/02/02 06:00
CRDT- 2013/03/01 06:00
PHST- 2014/09/14 00:00 [received]
PHST- 2014/09/28 00:00 [accepted]
PHST- 2013/03/01 06:00 [entrez]
PHST- 2013/03/01 06:00 [pubmed]
PHST- 2016/02/02 06:00 [medline]
AID - 1470320313478286 [pii]
AID - 10.1177/1470320313478286 [doi]
PST - ppublish
SO  - J Renin Angiotensin Aldosterone Syst. 2015 Mar;16(1):172-7. doi: 
      10.1177/1470320313478286. Epub 2013 Feb 27.