PMID- 23449129
OWN - NLM
STAT- MEDLINE
DCOM- 20130502
LR  - 20220310
IS  - 1525-7886 (Print)
IS  - 1525-7886 (Linking)
VI  - 64
IP  - 1
DP  - 2013 Jan-Feb
TI  - Effect and mechanism of epigallocatechin-3-gallate (EGCG). against the hydrogen 
      peroxide-induced oxidative damage in human dermal fibroblasts.
PG  - 35-44
AB  - This study was conducted to investigate the protective effects of 
      epigallocatechin-3-gallate (EGCG) on hydrogen peroxide (H2O2)-induced oxidative 
      stress injury in human dermal fibroblasts. 
      3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric 
      assay and the use of Hoechst staining and terminal deoxynucleotidyl transferase 
      dUTP nick end labeling for apoptosis detection indicated that the administration 
      of H2O2 to human dermal fibroblasts caused cell damage and apoptosis. The 
      incubation of human dermal fibroblasts with EGCG markedly inhibited the human 
      dermal fibroblast injury induced by H2O2. The assay for 
      2,2-diphenyl-1-picrylhydrazyl radical scavenging activity indicated that EGCG had 
      a direct, concentration-dependent antioxidant activity. Treatment of human dermal 
      fibroblasts with EGCG significantly reversed the H2O2-induced decrease of 
      superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), and the 
      inhibition of malondialdehyde (MDA) levels. These results showed that EGCG 
      possessed antioxidant activity and was effective against H2O2-induced human 
      dermal fibroblast injury by enhancing the activity of SOD and GSH-px, and by 
      decreasing the MDA level. Our results suggested that EGCG should have the 
      potential to be used further in cosmetics and in the prevention of aging-related 
      skin injuries.
FAU - Feng, Bing
AU  - Feng B
AD  - School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu, 
      People's Republic of China.
FAU - Fang, Yun
AU  - Fang Y
FAU - Wei, Shao-Min
AU  - Wei SM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cosmet Sci
JT  - Journal of cosmetic science
JID - 9814276
RN  - 0 (Benzimidazoles)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Picrates)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 8R1V1STN48 (Catechin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EUY85H477I (thiazolyl blue)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Benzimidazoles
MH  - Biphenyl Compounds
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cells, Cultured
MH  - Fibroblasts/*drug effects
MH  - Free Radical Scavengers/pharmacology
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/*adverse effects
MH  - In Situ Nick-End Labeling
MH  - Malondialdehyde
MH  - Oxidation-Reduction
MH  - Picrates
MH  - Skin/*cytology
MH  - Superoxide Dismutase/metabolism
MH  - Tetrazolium Salts/pharmacology
MH  - Thiazoles/pharmacology
EDAT- 2013/03/02 06:00
MHDA- 2013/05/03 06:00
CRDT- 2013/03/02 06:00
PHST- 2012/06/05 00:00 [accepted]
PHST- 2013/03/02 06:00 [entrez]
PHST- 2013/03/02 06:00 [pubmed]
PHST- 2013/05/03 06:00 [medline]
PST - ppublish
SO  - J Cosmet Sci. 2013 Jan-Feb;64(1):35-44.