PMID- 23450849
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20130814
IS  - 1934-6638 (Electronic)
IS  - 1934-662X (Linking)
VI  - 121
IP  - 8
DP  - 2013 Aug
TI  - Morphology of 9p21 homozygous deletion-positive pleural mesothelioma cells 
      analyzed using fluorescence in situ hybridization and virtual microscope system 
      in effusion cytology.
PG  - 415-22
LID - 10.1002/cncy.21269 [doi]
AB  - BACKGROUND: In malignant pleural mesothelioma (MPM), most patients first present 
      with pleural effusion; thus, cytologic analysis is the primary diagnostic 
      approach. However, the cytologic distinction between MPM and reactive mesothelial 
      cells (RMCs) in effusions can be extremely difficult due to the lack of both 
      well-established immunocytochemical markers and definite cytological criteria for 
      MPM. Moreover, the existence of both MPM cells and RMCs in effusions from the 
      same patient makes the differentiation even more challenging. Homozygous deletion 
      of the 9p21 locus, the site of the cyclin-dependent kinase inhibitor 2A/p16 
      (CDKN2A/p16) gene, frequently occurs in MPM but has never been reported in RMCs. 
      The aim of this study was to define the cytomorphological characteristics of MPM 
      cells, identified by the presence of 9p21 homozygous deletion by fluorescence in 
      situ hybridization (FISH). METHODS: For this purpose, cells on smear preparations 
      were recorded using a virtual microscope system and were subjected to FISH 
      analysis. Thereafter, 9p21 homozygous deletion-positive cells were identified in 
      the recorded virtual slides, followed by analysis of their morphological 
      characteristics. RESULTS: Mesothelioma cells positive for the 9p21 homozygous 
      deletion exhibited significantly more frequent cell-in-cell engulfment, 
      multinucleation (more than 2 nuclei), and larger multicellular clusters composed 
      of more than 10 cells than did 9p21 deletion-negative RMCs. Possible cutoff 
      values are also proposed for these morphological markers to differentiate MPM 
      cells from RMCs. CONCLUSIONS: These morphological differences and cutoff values 
      are useful for cytological differentiation of mesothelioma cells from RMCs. In 
      addition, the novel technique of a combination of virtual microscopy and FISH is 
      introduced for tumor morphological analysis.
CI  - Copyright (c) 2013 American Cancer Society.
FAU - Matsumoto, Shinji
AU  - Matsumoto S
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
FAU - Kamei, Toshiaki
AU  - Kamei T
FAU - Hiroshima, Kenzo
AU  - Hiroshima K
FAU - Kawahara, Kunimitsu
AU  - Kawahara K
FAU - Hata, Sakae
AU  - Hata S
FAU - Marukawa, Katsuji
AU  - Marukawa K
FAU - Matsuno, Yoshihiro
AU  - Matsuno Y
FAU - Taguchi, Kenichi
AU  - Taguchi K
FAU - Tsujimura, Tohru
AU  - Tsujimura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130228
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 9/genetics
MH  - Cytodiagnosis/*methods
MH  - Female
MH  - Genes, p16
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Mesothelioma/genetics/*pathology
MH  - Middle Aged
MH  - Pleural Effusion, Malignant/genetics/*pathology
MH  - Pleural Neoplasms/genetics/*pathology
MH  - *User-Computer Interface
OTO - NOTNLM
OT  - 9p21 homozygous deletion
OT  - cytology
OT  - fluorescence in situ hybridization
OT  - p16
OT  - pleural mesothelioma
EDAT- 2013/03/02 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/03/02 06:00
PHST- 2012/10/11 00:00 [received]
PHST- 2012/11/04 00:00 [revised]
PHST- 2012/11/19 00:00 [accepted]
PHST- 2013/03/02 06:00 [entrez]
PHST- 2013/03/02 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
AID - 10.1002/cncy.21269 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2013 Aug;121(8):415-22. doi: 10.1002/cncy.21269. Epub 2013 Feb 
      28.