PMID- 23451523
OWN - NLM
STAT- MEDLINE
DCOM- 20140402
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 11
DP  - 2012 Nov
TI  - [Simultaneous determination of eight derivatives of propranolol in cornea 
      perfusate in vitro by high performance liquid chromatography].
PG  - 1183-7
AB  - A high performance liquid chromatographic method was developed for the 
      simultaneous determination of eight derivatives of propranolol. Cassette dosing 
      method was used in the epithelium side of cornea in vitro to get the effect of 
      penetrant, and the perfusate was collected in the side of endothelium. The 
      protein in the sample was precipitated and discarded by high speed centrifugation 
      before injection. An Agilent Zorbax Extend column (150 mm x 3 mm, 5 microm) was 
      used at 30 degrees C. The mobile phase system contained acetonitrile and 0.03% 
      (v/v) phosphoric acid aqueous solution and the percentage of acetonitrile changed 
      between 3% and 20% (v/v) in a linear gradient elution. The samples were detected 
      by an ultraviolet (UV) detector at 205 nm. The results showed that the eight 
      derivatives of propranolol were completely separated and determined in 31 min. 
      The correlation coefficients were above 0.9970 and good linear relationships were 
      obtained in the range of 0.2 (0.1)-40.0 micromol/L. Under the optimized 
      conditions, the recoveries of the derivatives were in the range of 
      91.12%-105.73%. The intra-day relative standard deviations (RSDs) were in the 
      range of 1.00%-11.63%, and the inter-day RSDs were in the range of 1.18%-18.58%. 
      The sample showed stability under room temperature, freeze and three cycles of 
      freeze-thaw conditions. This method is fast and accurate for the quantitative 
      analysis of the derivatives of propranolol in transmembrane absorption such as 
      cornea perfusion in vitro or transwell cell system.
FAU - Wu, Haitao
AU  - Wu H
AD  - Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 
      Guangzhou 510405, China. znwht@sina.com.cn
FAU - Chen, Chuanbing
AU  - Chen C
FAU - Wang, Ningsheng
AU  - Wang N
FAU - Mi, Suiqing
AU  - Mi S
FAU - Liao, Nanying
AU  - Liao N
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 9Y8NXQ24VQ (Propranolol)
SB  - IM
MH  - Adrenergic beta-Antagonists/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cornea/*cytology/*metabolism
MH  - Humans
MH  - Perfusion
MH  - Propranolol/analogs & derivatives/*analysis
EDAT- 2013/03/05 06:00
MHDA- 2014/04/03 06:00
CRDT- 2013/03/05 06:00
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2014/04/03 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.06039 [doi]
PST - ppublish
SO  - Se Pu. 2012 Nov;30(11):1183-7. doi: 10.3724/sp.j.1123.2012.06039.