PMID- 23452217
OWN - NLM
STAT- MEDLINE
DCOM- 20130816
LR  - 20131121
IS  - 1532-4109 (Electronic)
IS  - 0360-1234 (Linking)
VI  - 48
IP  - 6
DP  - 2013
TI  - Acridine orange as an alternative to optical density to study growth kinetics of 
      Lactobacillus bulgaricus ATCC 7517.
PG  - 512-5
LID - 10.1080/03601234.2013.761919 [doi]
AB  - In this study we assessed the use of acridine orange as an alternative to optical 
      density to quantify the growth of Lactobacillus bulgaricus ATCC 7517. The growth 
      of bacteria in Lactobacillus de Man Rogosa Sharpe (MRS) medium was measured by 
      both acridine orange (AO) and optical density (OD) measurements for 24 h. The 
      relationship between both methods was compared via correlation analysis. The 
      doubling time of bacteria based on the values of OD600 and AO obtained during 
      24 h growth were also calculated. The result shows strong correlation of cell 
      growth between OD600 and AO during the first 10 hours of growth, but the 
      correlation was less strong when analyzing the data from 0 to 24 hours. Growth 
      rates, generation time and lag time were also similar. This study indicates that 
      AO could be used in place of OD to prepare growth curves of Lactobacillus 
      bulgaricus during the exponential phase of growth, and to compare growth rates, 
      generation times or lag times.
FAU - Pak, Dolar
AU  - Pak D
AD  - Center for Food Safety, Dept. of Food Science, University of Arkansas, 
      Fayetteville, Arkansas, USA.
FAU - Koo, Ok Kyung
AU  - Koo OK
FAU - Story, Robert S
AU  - Story RS
FAU - O'Bryan, Corliss A
AU  - O'Bryan CA
FAU - Crandall, Philip G
AU  - Crandall PG
FAU - Lee, Sun-Ok
AU  - Lee SO
FAU - Ricke, Steven C
AU  - Ricke SC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Kinetics
MH  - Lactobacillus/*chemistry/*growth & development
MH  - Spectrophotometry/*methods
MH  - Staining and Labeling/*methods
EDAT- 2013/03/05 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/03/05 06:00
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - 10.1080/03601234.2013.761919 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2013;48(6):512-5. doi: 10.1080/03601234.2013.761919.