PMID- 23452278
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20220310
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 74
IP  - 2
DP  - 2013 Apr
TI  - The cis-acting CTTC-P1BS module is indicative for gene function of LjVTI12, a 
      Qb-SNARE protein gene that is required for arbuscule formation in Lotus 
      japonicus.
PG  - 280-93
LID - 10.1111/tpj.12120 [doi]
AB  - The majority of land plants live in symbiosis with arbuscular mycorrhizal fungi 
      from the phylum Glomeromycota. This symbiosis improves acquisition of phosphorus 
      (P) by the host plant in exchange for carbohydrates, especially under low-P 
      availability. The symbiosome, constituted by root cortex cells accommodating 
      arbuscular mycorrhizal fungal hyphae, is the site at which bi-directional 
      exchange of nutrients and metabolites takes place. Uptake of orthophosphate (Pi) 
      in the symbiosome is facilitated by mycorrhiza-specific plant Pi transporters. 
      Modifications of the potato Pi transporter 3 (StPT3) promoter were analysed in 
      transgenic mycorrhizal roots, and it was found that the CTTC cis-regulatory 
      element is necessary and sufficient for a transcriptional response to fungal 
      colonization under low-Pi conditions. Phylogenetic footprinting also revealed 
      binary combination of the CTTC element with the Pi starvation response-associated 
      PHR1-binding site (P1BS) in the promoters of several mycorrhiza-specific Pi 
      transporter genes. Scanning of the Lotus japonicus genome for gene promoters 
      containing both cis-regulatory elements revealed a strong over-representation of 
      genes involved in transport processes. One of these, LjVTI12, encoding a member 
      of the SNARE family of proteins involved in membrane transport, exhibited 
      enhanced transcript levels in Lotus roots colonized with the arbuscular 
      mycorrhizal fungus Glomus intraradices. Down-regulation of LjVTI12 by RNA 
      interference resulted in a mycorrhiza-specific phenotype characterized by 
      distorted arbuscule morphology. The results highlight cooperative cis-regulation 
      which integrates mycorrhiza and Pi starvation signaling with vesicle trafficking 
      in symbiosome development.
CI  - (c) 2013 The Authors The Plant Journal (c) 2013 Blackwell Publishing Ltd.
FAU - Lota, Frederic
AU  - Lota F
AD  - Botanical Institute, Cologne Biocenter, Cluster of Excellence on Plant Sciences, 
      University of Cologne, Zulpicherstrasse 47b, D-50674, Cologne, Germany.
FAU - Wegmuller, Sarah
AU  - Wegmuller S
FAU - Buer, Benjamin
AU  - Buer B
FAU - Sato, Shusei
AU  - Sato S
FAU - Brautigam, Andrea
AU  - Brautigam A
FAU - Hanf, Benjamin
AU  - Hanf B
FAU - Bucher, Marcel
AU  - Bucher M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130316
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Lotus/genetics/*metabolism
MH  - Mycorrhizae/*physiology
MH  - Plant Proteins/genetics/*metabolism
MH  - RNA Interference
MH  - Solanum tuberosum/metabolism/microbiology
EDAT- 2013/03/05 06:00
MHDA- 2013/09/24 06:00
CRDT- 2013/03/05 06:00
PHST- 2012/11/24 00:00 [received]
PHST- 2013/01/08 00:00 [revised]
PHST- 2013/01/14 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
AID - 10.1111/tpj.12120 [doi]
PST - ppublish
SO  - Plant J. 2013 Apr;74(2):280-93. doi: 10.1111/tpj.12120. Epub 2013 Mar 16.